Frontiers in Plant Science (Apr 2022)

Control of Root Stem Cell Differentiation and Lateral Root Emergence by CLE16/17 Peptides in Arabidopsis

  • Lihua Zhang,
  • Yi Yang,
  • Changqing Mu,
  • Mingyu Liu,
  • Takashi Ishida,
  • Takashi Ishida,
  • Shinichiro Sawa,
  • Yuxian Zhu,
  • Yuxian Zhu,
  • Limin Pi

DOI
https://doi.org/10.3389/fpls.2022.869888
Journal volume & issue
Vol. 13

Abstract

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Secreted peptide-mediated cell-to-cell communication plays a crucial role in the development of multicellular organisms. A large number of secreted peptides have been predicated by bioinformatic approaches in plants. However, only a few of them have been functionally characterized. In this study, we show that two CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides CLE16/17 are required for both stem cell differentiation and lateral root (LR) emergence in Arabidopsis. We further demonstrate that the CLE16/17 peptides act through the CLAVATA1-ARABIDOPSIS CRINKLY4 (CLV1-ACR4) protein kinase complex in columella stem cell (CSC) differentiation, but not in LR emergence. Furthermore, we show that CLE16/17 promote LR emergence probably via activating the expression of HAESA/HAESA-LIKE2 (HAE/HSL2) required for cell wall remodeling. Collectively, our results reveal a CLV1-ACR4-dependent and -independent dual-function of the CLE16/17 peptides in root development.

Keywords