Frontiers in Cell and Developmental Biology (Sep 2021)
Single-Cell RNA Sequencing Analysis Reveals Greater Epithelial Ridge Cells Degeneration During Postnatal Development of Cochlea in Rats
- Jianyong Chen,
- Jianyong Chen,
- Jianyong Chen,
- Dekun Gao,
- Dekun Gao,
- Dekun Gao,
- Junmin Chen,
- Junmin Chen,
- Junmin Chen,
- Shule Hou,
- Shule Hou,
- Shule Hou,
- Baihui He,
- Baihui He,
- Baihui He,
- Yue Li,
- Yue Li,
- Yue Li,
- Shuna Li,
- Shuna Li,
- Shuna Li,
- Fan Zhang,
- Fan Zhang,
- Fan Zhang,
- Xiayu Sun,
- Xiayu Sun,
- Xiayu Sun,
- Fabio Mammano,
- Fabio Mammano,
- Lianhua Sun,
- Lianhua Sun,
- Lianhua Sun,
- Jun Yang,
- Jun Yang,
- Jun Yang,
- Guiliang Zheng,
- Guiliang Zheng,
- Guiliang Zheng
Affiliations
- Jianyong Chen
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Jianyong Chen
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Jianyong Chen
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Dekun Gao
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Dekun Gao
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Dekun Gao
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Junmin Chen
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Junmin Chen
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Junmin Chen
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Shule Hou
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Shule Hou
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Shule Hou
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Baihui He
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Baihui He
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Baihui He
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Yue Li
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Yue Li
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Yue Li
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Shuna Li
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Shuna Li
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Shuna Li
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Fan Zhang
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Fan Zhang
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Fan Zhang
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Xiayu Sun
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Xiayu Sun
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Xiayu Sun
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Fabio Mammano
- Department of Physics and Astronomy “G. Galilei”, University of Padova, Padua, Italy
- Fabio Mammano
- Department of Biomedical Sciences, Institute of Cell Biology and Neurobiology, Italian National Research Council, Monterotondo, Italy
- Lianhua Sun
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Lianhua Sun
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Lianhua Sun
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Jun Yang
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Jun Yang
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Jun Yang
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- Guiliang Zheng
- Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
- Guiliang Zheng
- Shanghai Jiao Tong University School of Medicine Ear Institute, Shanghai, China
- Guiliang Zheng
- Shanghai Key Laboratory of Translational Medicine on Ear and Nose Diseases, Shanghai, China
- DOI
- https://doi.org/10.3389/fcell.2021.719491
- Journal volume & issue
-
Vol. 9
Abstract
Greater epithelial ridge cells, a transient neonatal cell group in the cochlear duct, which plays a crucial role in the functional maturation of hair cell, structural development of tectorial membrane, and refinement of audio localization before hearing. Greater epithelial ridge cells are methodologically homogeneous, while whether different cell subtypes are existence in this intriguing region and the degeneration mechanism during postnatal cochlear development are poorly understood. In the present study, single-cell RNA sequencing was performed on the cochlear duct of postnatal rats at day 1 (P1) and day 7 (P7) to identify subsets of greater epithelial ridge cell and progression. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were used to examine genes enriched biological processes in these clusters. We identified a total of 26 clusters at P1 and P7 rats and found that the cell number of five cell clusters decreased significantly, while four clusters had similar gene expression patterns and biological properties. The genes of these four cell populations were mainly enriched in Ribosome and P13K-Akt signal pathway. Among them, Rps16, Rpsa, Col4a2, Col6a2, Ctsk, and Jun are particularly interesting as their expression might contribute to the greater epithelial ridge cells degeneration. In conclusion, our study provides an important reference resource of greater epithelial ridge cells landscape and mechanism insights for further understanding greater epithelial ridge cells degeneration during postnatal rat cochlear development.
Keywords
