Modulatory efficacy of Punica granatum L. powder ethanol extract (PLEE) on lead acetate-induced hepatic and renal toxicity

Abstract

Abstract Background Pomegranate powder derived from pomegranate fruit contains phytochemicals chief of which is ellagitannins. Owing to their high antioxidative capabilities we investigated its efficacy against lead acetate induced hepatic and renal toxicity. Methods Pomegranate powder (500 g) was procured and soaked in 1 Litre of ethanol for three days, its filtrate was concentrated and freeze dried to form Punica granatum L. ethanol extract (PLEE). Thirty (30) male albino Wistar rats with average weight between 140 ± 20 g were used for this study. They were equally shared into 6 groups with 5 animals each; A: Control; B: Lead acetate only C: Lead acetate + PLEE (1); D: Lead acetate + PLEE (2); E: PLEE (1) and F: PLEE (2). Serum, liver and kidney samples were obtained for biochemical assays. The integrity of liver and kidney tissues were also accessed. Results Lead administration resulted in marked elevations in liver transaminases in serum and liver as well as a significant increase in kidney function markers in the serum and kidney (p < 0.05). There was also a significant decline in average body weight, antioxidant markers in the liver and kidney of Wistar rats while there was a significant increase in lipid peroxidation levels in both liver and kidney. However, administration of PLEE at different doses significantly modulated these changes. Conclusion Lead acetate administration led to hepatic and renal toxicity as evident by alterations in liver and kidney function markers, antioxidant status and tissue integrity. However, PLEE can combat these toxicities.

Keywords

• Lead acetate
• Punica granatum L.
• Pomegranate powder
• Hepatotoxicity
• Renal toxicity
• Oxidative stress