Epigenetics (Dec 2023)

Suppressing circIDE/miR-19b-3p/RBMS1 axis exhibits promoting-tumour activity through upregulating GPX4 to diminish ferroptosis in hepatocellular carcinoma

  • Hang Zhai,
  • Sisi Zhong,
  • Runxin Wu,
  • Zhaohong Mo,
  • Shiyang Zheng,
  • Jinhua Xue,
  • Hongyu Meng,
  • Maosheng Liu,
  • Xianyu Chen,
  • Guangquan Zhang,
  • Xiyan Zheng,
  • Fei Du,
  • Ruixi Li,
  • Boxuan Zhou

DOI
https://doi.org/10.1080/15592294.2023.2192438
Journal volume & issue
Vol. 18, no. 1

Abstract

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Ferroptosis is a newly characterized form of iron-dependent non-apoptotic cell death, which is closely associated with cancer progression. However, the functions and mechanisms in regulation of escaping from ferroptosis during hepatocellular carcinoma (HCC) progression remain unknown. In this study, we reported that the RNA binding motif single stranded interacting protein 1 (RBMS1) participated in HCC development,and functioned as a regulator of ferroptosis. Clinically, the downregulation of RBMS1 occurred in HCC tissues, and low RBMS1 expression was associated with worse HCC patients survival. Mechanistically, RBMS1 overexpression inhibited HCC cell growth by attenuating the expression of glutathione peroxidase 4 (GPX4)and further facilitated ferroptosis in vitro and in vivo. More importantly, a novel circIDE (hsa_circ_0000251) was identified to elevate RBMS1 expression via sponging miR-19b-3p in HCC cells. Collectively, our findings established circIDE/miR-19b-3p/RBMS1 axis as a regulator of ferroptosis, which could be a promising therapeutic target and prognostic factor.

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