The Application of Clinical Genetics (Oct 2019)
Genetic Analysis Of ABCA1 Gene Of Primary Glaucoma In Jordanian Arab Population
Abstract
Rami Alkhatib,1 Nada Abudhaim,1 Laith AL-Eitan,2 Nour Abdo,3 Asem Alqudah,4 Hatem Aman1 1Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid 22110, Jordan; 2Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan; 3Department of Public Health, Faculty of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan; 4Department of Ophthalmology, Faculty of Medicine, Jordan University of Science and Technology, Irbid 22110, JordanCorrespondence: Rami AlkhatibDepartment of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid 22110, JordanTel +9622776319493Email [email protected]: Glaucoma is a neurodegenerative disease that leads to progressive loss of retinal ganglion cells, causing irreversible visual field defects. At the present time, glaucoma is clinically defined but the exact etiology is unknown. The aim of this study is to genotype rs2472493 and rs2487032 SNIPs within ABCA1 gene in 52 Jordanian Arab patients with primary glaucoma and 96 control subjects, and also to investigate the genetic association of these SNPs with primary glaucoma.Methods: DNA was extracted from both patients and controls according to a well-established procedure. Then, DNA was amplified by PCR using specific primers for this gene. Analysis of polymorphisms was carried out by using DNA sequencing genotyping method.Results: The results showed that the two SNPs (rs2472493 and rs2487032) located upstream of ABCA1 gene have no significant associations with primary glaucoma disorder (P > 0.05).Conclusion: This study is the first of its kind to reveal no genetic association between ABCA1 gene and primary glaucoma disorder in Jordanian population of Arab descent.Keywords: ABCA1, glaucoma, polymorphisms, Jordanian Arab, primers
