HinfI Polymorphisms Characterization in the 1st intron of BMP15/FecX Gene in Turcana and Tsigai Sheep (Ovis aries)

Abstract

BMP15-FecX is one of the major genes associated with prolificacy in different sheep breeds (Ovis aries), alongside BMPR15/FecB and GDF9/FecG. The majority of the polymorphisms, associated with ovulation rate in different breeds, are located in the exonic sequences of the gene. In the present study, a fragment of 356 bp, adjacent to the 2nd exon of the BMP-15 gene, was amplified by PCR in 50 individuals of Turcana and Tsigai breeds. The amplified products were subjected to digestion to identify the polymorphisms in the HinfI restriction enzyme sites. The two putative enzyme sites presented in the fragment did not reveal mutations in neither breed, releasing the same electrophoretic profile.

Keywords

• snp
• polymorphism
• bmp15 gene
• restriction enzyme