Designing of novel chimeric PvpA-pMGA protein of Mycoplasma gallisepticum, applicable for indirect ELISA

Farzaneh pourkarimi Fatideh; Majid Esmaelizad; Mohammad Kargar; Majid Tebianian; Farshid Kafilzadeh

doi:10.1186/s43141-022-00434-0

Journal of Genetic Engineering and Biotechnology (Nov 2022)

Designing of novel chimeric PvpA-pMGA protein of Mycoplasma gallisepticum, applicable for indirect ELISA

Farzaneh pourkarimi Fatideh,
Majid Esmaelizad,
Mohammad Kargar,
Majid Tebianian,
Farshid Kafilzadeh

Affiliations

Farzaneh pourkarimi Fatideh: Department of Microbiology, Jahrom Branch, Islamic Azad University
Majid Esmaelizad: Research and Development Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO)
Mohammad Kargar: Department of Microbiology, Jahrom Branch, Islamic Azad University
Majid Tebianian: Department of Immunology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO)
Farshid Kafilzadeh: Department of Microbiology, Jahrom Branch, Islamic Azad University

DOI: https://doi.org/10.1186/s43141-022-00434-0
Journal volume & issue: Vol. 20, no. 1
pp. 1 – 12

Abstract

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Highlights • This is first study to present chimeric protein rPvpA-pMGA as a candidate antigen in ELISA. • High-yield 138 mg/L purification of recombinant protein. • rPvpA-pMGA is a reliable immunogenic protein which induced high amount of humoral immune response. • rPvpA-pMGA showed high performance for evaluation of antibody level in vaccinated poultry flocks.

Published in Journal of Genetic Engineering and Biotechnology

ISSN: 1687-157X (Print); 2090-5920 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Biology (General): Genetics
Website: https://www.sciencedirect.com/journal/journal-of-genetic-engineering-and-biotechnology

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Abstract

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