DETERMINATION OF PROTEINS FROM BUFFALO MILK USING HIGH PERFORMANCE LIQUID CROMATOGRAPHY RP-HPLC

Abstract

In the hereby paper, we have undertaken a study on buffalo milk proteins, employing high performance liquid chromatography (HPLC). This RP-HPLC technique is commonly employed in the separation and assessment of caseins K, and in the fresh, as well as processed milk. These methods are also successfully applied in the authenticity and origin assessment of certain cheese products and the qualitative analysis of milk in bubalines, ovines, caprines and bovines (Ferreira şi Cacote, 2003; Veloso şi colab., 2002). In order to identify the main protein types, we found support in literature data (Miranda şi colab., 2004), and thus achieved the chromatographic separation on whole milk, lactoserum and casein samples.

Keywords

• buffalo
• proteins
• hplc