Association of tumor necrosis factor-β+252 gene polymorphism and H.pylori infection with the susceptibility to non-cardiac gastric cancer

Abstract

Objective To evaluate the association of tumor necrosis factor-β (TNF-β) +252 gene polymorphisms and H.pylori (Hp) infection with the susceptibility to non-cardiac gastric cancer. Methods A total of 215 patients with non-cardiac cancer and 215 healthy control subjects were recruited from the First Affiliated Hospital of Fujian Medical University between March, 2015 and December, 2018. Pathological typing of gastric cancer was performed according to Lauren's criteria, and the correlation of TNF-β+252 genotypes and Hp infection with the clinicopathological characteristics of the patients was analyzed. Results Compared with Hp-negative patients, Hp-positive patients had a significantly increased risk for gastric cancer (OR=1.881, 95%CI: 1.277-2.770). Compared with G/G genotype of TNF-β +252, the genotypes G/A and A/A were associated with significantly increased risks for gastric cancer. In the control group, no significant difference was found in the genotype distribution between the Hp-negative and Hp-positive subjects (Chi-square=2.801, P=0.246). In the case group, the proportion of G/A and A/A genotypes was significantly greater in Hp-positive patients than in Hp-negative patients (Chi-square=6.265, P=0.044). The G/A+A/A genotype frequencies were significantly higher in patients with intestinal gastric cancer than in the control group (Chi-square=11.325, P=0.003). There was an interaction between the allele A in TNF-β gene +252 and Hp infection. Conclusion Hp infection increases the susceptibility to non-cardiac gastric cancer, which is also associated with TNF-β gene +252 G/A and A/A genotypes.

Keywords

• non-cardiac gastric cancer
• tumor necrosis factor-β
• gene polymorphisms
• helicobacter pylori infection