Colorimetric RT-LAMP and LAMP-sequencing for Detecting SARS-CoV-2 RNA in Clinical Samples
Konrad Herbst,
Matthias Meurer,
Daniel Kirrmaier,
Simon Anders,
Michael Knop,
Viet Dao Thi
Affiliations
Konrad Herbst
Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany
Matthias Meurer
Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany
Daniel Kirrmaier
German Cancer Research Center (DKFZ), Heidelberg, Germany
Simon Anders
Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany
Michael Knop
Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, GermanyGerman Cancer Research Center (DKFZ), Heidelberg, Germany, DKFZ-ZMBH Alliance, Heidelberg, Germany
Viet Dao Thi
Schaller Research Group, Department of Infectious Diseases, Virology, Heidelberg University, Heidelberg, Germany
During pandemics, such as the one caused by SARS-CoV-2 coronavirus, simple methods to rapidly test large numbers of people are needed. As a faster and less resource-demanding alternative to detect viral RNA by conventional qPCR, we used reverse transcription loop-mediated isothermal amplification (RT-LAMP). We previously established colorimetric RT-LAMP assays on both purified and unpurified SARS-CoV-2 clinical specimens and further developed a multiplexed sequencing protocol (LAMP-sequencing) to analyze the outcome of many RT-LAMP reactions at the same time (Dao Thi et al., 2020). Extending on this work, we hereby provide step-by-step protocols for both RT-LAMP assays and read-outs.
