Potential therapeutic effect of synthesized AgNP using curcumin extract on CCl4-induced nephrotoxicity in male mice

Abstract

Nephrotoxicity is a particularly important challenge in a clinical context. Oxidative stress, plays an important role in the pathogenesis of kidney. Curcumin has a potential antioxidant impacts, despite of its low water solubility and bioavailability which restricts its uptake. Here, we elevated curcumin solubilization and bioactivity by including in a modified silver nanoparticles. This study is aimed at assessing the protective impacts of silver nanoparticles with curcumin (AgNP-curcumin) against CCl4-induced injury in mice kidney. C57BL/6 mice were categorized into three groups. Group 1 mice received vehicle only. Group 2 mice received a single dose of 1 mL/kg CCl4 in paraffin. Group 3 mice were treated with 2.5 mg/kg AgNP-curcumin twice a week for 3 weeks after receiving CCl4 challenge. Results showed that CCl4 disrupted oxidative markers. Reduced glutathione was significantly decreased and the lipid peroxidation indicator MDA was significantly elevated in the CCl4 group. Urea, creatinine and gamma glutamyl transferase (GGT) were significantly elevated in the CCl4 group. However, lactate dehydrogenase (LDH) was significantly decreased. Severe histopathological changes were observed in the renal tissues in the CCl4 group compared with the findings in the control group. AgNP-curcumin was found to significantly restore the oxidative balance and kidney structure and function. The comet assay showed that CCl4 increases nuclear DNA tail length (DNA damage) by 76.36% compared with the control, while AgNP-curcumin demonstrated a decrease in tail length by 30.53% relative to that in the CCl4 group. Through its antioxidant activity, AgNP-curcumin showed a potential role in preventing the deleterious effects of CCl4 on DNA in renal tissues.

Keywords

• Carbon tetrachloride
• Nephrotoxicity
• Oxidative stress
• Toxicity markers
• Silver nanoparticles-curcumin