Respiratory burst in neutrophilic granulocytes of carps (Cyprinus carpio): cytometric studies

Abstract

Present studies aimed at flow cytometric analysis of respiratory burst in neutrophilic granulocytes in carps, at various stages of their ontogeny. Cytometric evaluation of the repiratory burst in PMN cells of carps demonstrated augmented values of the mean fluorescence channel of PMA-stimulated PMN cells, as compared to the value of the fluorescence channel noted for PMN cells which were not activated or did not respond to activation in response to the applied stimulator. Intensity of the fluorescence, compared to the mean fluorescence channel of the cells which were not stimulated by PMA, was most pronounced in 11- to 21-month old carps, average in the youngest carps aging 3 to 9 months, and the lowest in the eldest, 23- to 29-month old carps. In cases of analysis of the mean fluorescence channel in the selected fraction of PMA-stimulated PMN cells, as compared to the mean fluorescence channel of granulocytes which did not respond by the stimulation reaction, the most intense fluorescence was noted to develop in the eldest, i.e. 23- to 29-month old fish. In the remaining carps, on the other hand, the difference in the mean fluorescence channel was significantly lower. In parallel, the highest fraction of PMA-stimulated granulocytes was found in 11- to 21-month old carps, a lower fraction in the youngest carps aging 3 to 9 months and the lowest fraction of such granulocytes in the eldest carps, 23 to 29 months of age.

Keywords

• carp
• pmn cells
• respiratory burst
• citometry