Silencing of a Germin-Like Protein Gene (CchGLP) in Geminivirus-Resistant Pepper (Capsicum chinense Jacq.) BG-3821 Increases Susceptibility to Single and Mixed Infections by Geminiviruses PHYVV and PepGMV
Laura Mejía-Teniente,
Ahuizolt de Jesús Joaquin-Ramos,
Irineo Torres-Pacheco,
Rafael F. Rivera-Bustamante,
Lorenzo Guevara-Olvera,
Enrique Rico-García,
Ramon G. Guevara-Gonzalez
Affiliations
Laura Mejía-Teniente
C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico
Ahuizolt de Jesús Joaquin-Ramos
Instituto Tecnológico de Roque, Departamento de Ingeniería en Industrias Alimentarias, Km. 8 Carr. Celaya-J. Rosas, Roque, Celaya, Gto C.P. 38110, Mexico
Irineo Torres-Pacheco
C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico
Rafael F. Rivera-Bustamante
Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados (CINVESTAV)-Unidad Irapuato, Carretera Irapuato-Leon, Km 9.6, Libramiento norte, Irapuato, Guanajuato A.P. 629, Mexico
Lorenzo Guevara-Olvera
Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Ave. Tecnológico y A, Garcia-Cubas, S/N, Col. FOVISSSTE, Celaya, Gto A.P. 57, Mexico
Enrique Rico-García
C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico
Ramon G. Guevara-Gonzalez
C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico
Germin-like proteins (GLPs) are encoded by a family of genes found in all plants, and in terms of function, the GLPs are implicated in the response of plants to biotic and abiotic stresses. CchGLP is a gene encoding a GLP identified in a geminivirus-resistant Capsicum chinense Jacq accession named BG-3821, and it is important in geminivirus resistance when transferred to susceptible tobacco in transgenic experiments. To characterize the role of this GLP in geminivirus resistance in the original accession from which this gene was identified, this work aimed at demonstrating the possible role of CchGLP in resistance to geminiviruses in Capsicum chinense Jacq. BG-3821. Virus-induced gene silencing studies using a geminiviral vector based in PHYVV component A, displaying that silencing of CchGLP in accession BG-3821, increased susceptibility to geminivirus single and mixed infections. These results suggested that CchGLP is an important factor for geminivirus resistance in C. chinense BG-3821 accession.
