International Brazilian Journal of Urology (Dec 2012)

[Article title missing]

  • Paulo Ornellas,
  • Antonio Augusto Ornellas,
  • Clizia Chinello,
  • Erica Gianazza,
  • Veronica Mainini,
  • Marta Cazzaniga,
  • Denise Abreu Pereira,
  • Vanessa Sandim,
  • Ana Sheila Cypriano,
  • Leandro Koifman,
  • Paulo Cesar Barbosa da Silva,
  • Gilda Alves,
  • Fulvio Magni

Journal volume & issue
Vol. 38, no. 6
pp. 739 – 749

Abstract

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PurposeTo investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP).Materials and MethodsPlasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identified from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS.ResultsA cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specificity, respectively. The cluster also showed very high sensitivity (100%) and specificity (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specificity of 80% and 97%, respectively. These two peptides were identified by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins.ConclusionsThe results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools.

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