Antimicrobial Effect of Extracts of Daphne Oleoides on Bacteria Isolated from Dental Plaque

Abstract

Introduction: Oral cavity a favorable environment for the growth and colonization of a wide range of bacteria. Plaques or dental biofilms are a wide variety of bacteria found on a tooth surface. The aim of the present study is to investigate the antibacterial effect of Daphne Oleoides on bacteria isolated from dental plaque. Materials and Methods: In this cross-sectional study, after the collection of dental plaques and isolation of bacteria, bacteria were identified using biochemical tests and Polymerase chain reaction (PCR). Leaves and stems of D. oleoides were collected in the growing season and dried in shadows. The aqueous, alcoholic and hydroalcoholic extracts of D. oleoides were withdrawn using the classical extraction method. Thereafter, its antimicrobial effects on bacteria isolated from dental plaque were examined using the disk diffusion method and minimum inhibitory concentration (MIC). Data were analyzed using Turkey’s and Anova test (PResults: The obtained results of the present study confirmed the presence of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Streptococcus mutans in the collected dental plaques. The alcoholic extract demonstrated its highest antimicrobial activity against S. mutans with 20.55±1.05 mm inhibition zone and a Minimum lethal concentration (MLC) of 0.19 mg/ml. On the other hand, the lowest antimicrobial activity of the alcoholic extract was reported to be against S. epidermidis with 7.4±0.5 mm inhibition zone and MLC 12.5 mg/ml. Conclusion: The results of the current study indicated that the alcoholic extract of D. oleoides is able to withdraw antibacterial compounds on the bacteria which are responsible for oral infections.

Keywords

• dental plaque
• minimum inhibitory concentration (mic)
• daphne oleoides