Effect of Music on the Decreased Enamel-Surface Mineral Content of Rat Teeth with Protein Deficiency

Abstract

Protein deficiency could lead to enamel hypoplasia and decreased level of Growth Hormone (GH). Cell proliferation and synthesis of enamel-matrix which affect the mineralization process of the tissue, are stimulated by GH. Music was reported to be able to increase GH. Objective: Analyzing the effect of music exposure since prenatal on the decreased Calcium (Ca) and Phosphor (P) content of the enamel-surface of rat-pups in protein-deficiency condition. Experiment: Thirty-two rats on the first day of gestation period were divided into groups with and without music. Music were given twice daily, lullabies every early morning, and classic, baroc, and romantic music every evening. At 2-days-old the rat-pups were further divided into groups with normal diet contained 19.5% protein, and groups with protein deficiency diet contained 7.5% protein. At 2- and 5-weeks-old, 6 rat pups from each group were randomly terminated, the mandibles were dissected out, cut into hemi-mandibles, cleaned, and dried. The percentage of Ca and P content of the lower-incisor enamel-surface was analyzed using Energy Dispersive X-ray (EDX), data were analyzed using One Way ANOVA with α 0.05. Results: At 2-weeks-old, the Ca (8.6%) and P (10.6%) contents of enamel-surface of pups with normal-diet and music were higher than the Ca (3.9%) and P(7.9%) contents of enamel-surface of pups with protein-deficiency with no music (P<0.029). Among 5-weeks-old pups with protein-deficiency, the P content (6.1%) of enamel-surface of pups with music were higher than P content (2.8%) of enamel-surface of pups with no music (P<0.034). Conclusion: Music has a potency to minimize the decreased Ca and P enamel content on the protein deficiency condition.

Keywords

• enamel
• mineral
• music
• protein-deficiency