Formulation and Evaluation of Amikacin Sulfate Loaded Dextran Nanoparticles against Human Pathogenic Bacteria
Rahamat Unissa Syed,
Sivakumar S. Moni,
Muhammad Nawaz,
Mohammed Khaled Bin Break,
Nasrin E. Khalifa,
Siddig Ibrahim Abdelwahab,
Reham Meshal Alharbi,
Raghad Huraid Alfaisal,
Bayan Naif Al Basher,
Entsar Mohammed Alhaidan
Affiliations
Rahamat Unissa Syed
Department of Pharmaceutics, College of Pharmacy, University of Ha’il, Hail 81442, Saudi Arabia
Sivakumar S. Moni
Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia
Muhammad Nawaz
Department of Nano-Medicine Research, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia
Mohammed Khaled Bin Break
Medical and Diagnostic Research Centre, University of Ha’il, Hail 55473, Saudi Arabia
Nasrin E. Khalifa
Department of Pharmaceutics, College of Pharmacy, University of Ha’il, Hail 81442, Saudi Arabia
Siddig Ibrahim Abdelwahab
Medical Research Center, Jazan University, Jazan 45142, Saudi Arabia
Reham Meshal Alharbi
College of Pharmacy, University of Ha’il, Hail 81442, Saudi Arabia
Raghad Huraid Alfaisal
College of Pharmacy, University of Ha’il, Hail 81442, Saudi Arabia
Bayan Naif Al Basher
College of Pharmacy, University of Ha’il, Hail 81442, Saudi Arabia
Entsar Mohammed Alhaidan
College of Pharmacy, University of Ha’il, Hail 81442, Saudi Arabia
Amikacin sulfate-loaded dextran sulfate sodium nanoparticles were formulated, lyophilized (LADNP), and then analyzed. The LADNP had a −20.9 ± 8.35 mV zeta potential, PDI of 0.256, and % PDI of 67.7. The zeta average nano size of LADNP was 317.9 z. d.nm, while the dimension of an individual particle was 259.3 ± 73.52 nm, and nanoparticle conductivity in colloidal solution was 2.36 mS/cm. LADNP has distinct endothermic peaks at temperatures at 165.77 °C, according to differential scanning calorimetry (DSC). The thermogravimetric analysis (TGA) showed the weight loss of LADNP, which was observed as 95% at 210.78 °C. XRD investigation on LADNP exhibited distinct peaks at 2θ as 9.6°, 10.4°, 11.4°, 18.9°, 20.3°, 24.4°, 28.2°, 33.2°, 38.9°, and 40.4° confirming crystalline structure. The amikacin release kinetics from LADNP revealed zero order kinetics with a linear release showed zero order kinetics with 37% of drug release in 7 h and had an R2 value of 0.99. The antibacterial effect of LADNP showed broad-spectrum activity against tested human pathogenic bacteria. The preset study demonstrated that LADNP is a promising antibacterial agent.
