Role of LncRNA H19 in promoting endometrial deciduation

Abstract

Objective To explore the effect of LncRNA H19 on endometrial decidualization. Methods The expression of LncRNA H19 in decidual tissues of spontaneous abortion and induced abortion was detected by RT-qPCR; The expression of LncRNA H19 in non-pregnant endometrium and decidual tissues of different gestational weeks was detected; Using MPA and 8-Br-cAMP inducers to induce the transformed human endometrial stromal cells (THESCs), RT-qPCR was used to detect the expression of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP-1); The expression changes of LncRNA H19 and FOXO1 were detected after the process of decidualization; Lentivirus was used to overexpress and interfere with LncRNA H19 in THESCs, and the proliferation of THESCs was detected by CCK-8 assay. After successful lentivirus transfection, decidualization was induced. RT-qPCR was used to detect the expression of PRL, IGFBP-1 and FOXO1 in blank control group, LV-GFP induction group, LV-H19-RNAi induction group and LV-H19 induction group. Results The expression of LncRNA H19 in spontaneous abortion group was lower than that in induced abortion group (P < 0.01); LncRNA H19 reached its peak at the 7th week of decidual tissue in early pregnancy (P < 0.01); After the decidualization of THESCs, the levels of PRL, IGFBP-1, FOXO1 and LncRNA H19 were higher than those of the control group (P < 0.01).The results of CCK-8 assay showed that the proliferation of THESCs was inhibited after the interference with LncRNA H19 (P < 0.01), while the proliferation of THESCs was promoted after the overexpression of LncRNA H19 (P < 0.01). The decidualization induction was performed, after interfering with and overexpressing the LncRNA H19 of THESCs. Compared with the blank control group, the LV-GFP induction group showed the increased mRNA expression of PRL (P < 0.05), IGFBP-1 (P < 0.01) and FOXO1 (P < 0.01). Compared with LV-GFP induction group, the mRNA expression of PRL (P < 0.05) and IGFBP-1 (P < 0.01) in LV-H19 induction group was increased, while the mRNA expression of PRL (P < 0.05), IGFBP-1 (P < 0.01) and FOXO1 (P < 0.01) in LV-H19-RNAi induction group was decreased. Conclusion LncRNA H19 promotes the proliferation and decidualization of endometrial stromal cells.

Keywords

• lncrna h19
• foxo1
• decidualization
• spontaneous abortion