Genetic analysis and phylogenetic relationships of the Barb horse through the use of microsatellites

Abstract

The aim of this study was to compare genetically Moroccan (MA) and Tunisian (TN) Barb horses and toe stablish the phylogenic relation between Barb horse and other horse breeds in Morocco by using microsatellites. First, 100 Moroccan barb and 100 Tunisian Barb horses were used. Genetic variability index between and inside the populations were estimated. 133 alleles were detected in the Moroccan population and 125 in the Tunisian. The mean number of alleles was 7.29 and 7.82 respectively for the Tunisian and Moroccan barb horse. The heterozygosity was almost similar in the two groups of Barb horses (TN = 0.716 and MA = 0.714). Fst was estimated at 0.0454 and 0.0780 respectively for TN and MA Barb horses. The Gst was also estimated at 0.012 and the Nei genetic distance at 0.05. The two populations are almost genetically similar and heterogeneous. Finally, 86,5 % of horses were correctly affected to their likelihood population. Secondly, 50 Arabian horses, 50 Arabian-Barb and 50 thoroughbreds and the 200 Barb horses previously studied were used to establish the phylogenic relationships. The use of 17 microsatellites showed that the Barb horses had specific alleles and the highest heterozygosity and the highest genetic distances were obtained with thoroughbred. The two Barb horse populations were highly combined in one side of the phylogenic tree.

Keywords

• horse
• barb
• genetic analysis
• microsatellites
• genetic index
• phylogenic tree