The role of CD83 in the pathogenesis of immune thrombocytopenia
Xiuli Wang,
Qiyuan Zhou,
Wen Yang,
Hui Bi,
Honghui Wang,
Yacan Wang,
Yadong Du,
Lin Liu,
Yuebo Liu,
Liefen Yin,
Jin Yao,
Jingxing Yu,
Wei Tao,
Yongchun Zhou,
Zeping Zhou
Affiliations
Xiuli Wang
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Qiyuan Zhou
Department of Molecular and Cellular Biology, University of California, Davis, USA
Wen Yang
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Hui Bi
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Honghui Wang
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Yacan Wang
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Yadong Du
Department of Hematology, The First Affiliated Hospital of Henan University of Science and Technology, Henan, People's Republic of China
Lin Liu
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Yuebo Liu
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Liefen Yin
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Jin Yao
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Jingxing Yu
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Wei Tao
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Yongchun Zhou
Department of Molecular Diagnostic Center, Kunming Medical University, School of Clinical Oncology: Yunnan Cancer Hospital, Kunming, People’s Republic of China
Zeping Zhou
Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming, People’s Republic of China
Background CD83 are closely related to the pathogenesis of immune thrombocytopenia (ITP), but the exact mechanism remains unclear.Aim To explore the relationship between CD83 and CD4+ T cell subsets and clarify the role of CD83 in the pathogenesis of ITP.Methods RT-qPCR and Flow cytometry were used to illustrate CD83 expression. The downregulation and overexpression of DC-CD83 were co-cultured with CD4+ T cells to detect cell proliferation, co-cultured supernatant cytokines and Tregs expression.Results The results indicate that the ITP patients showed higher expression of CD83 than the healthy controls. The proliferation of CD4+ T cells was inhibited by downregulation of DCs-CD83 but promoted by overexpression of DCs-CD83. siRNA-CD83 inhibited proinflammatory IFN-γ and IL-17 secretion while raising TGF-β, IL-10 concentrations. Overexpression of DCs-CD83 promoted Tregs expression.Conclusion The Th1/Th2 and Th17/Tregs polarization were reversed via interfering DCs with siRNA-CD83. CD83 plays an important role in ITP pathogenesis, suggesting novel treatment for ITP patients.