Hematology (Dec 2024)

The role of CD83 in the pathogenesis of immune thrombocytopenia

  • Xiuli Wang,
  • Qiyuan Zhou,
  • Wen Yang,
  • Hui Bi,
  • Honghui Wang,
  • Yacan Wang,
  • Yadong Du,
  • Lin Liu,
  • Yuebo Liu,
  • Liefen Yin,
  • Jin Yao,
  • Jingxing Yu,
  • Wei Tao,
  • Yongchun Zhou,
  • Zeping Zhou

DOI
https://doi.org/10.1080/16078454.2024.2372482
Journal volume & issue
Vol. 29, no. 1

Abstract

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Background CD83 are closely related to the pathogenesis of immune thrombocytopenia (ITP), but the exact mechanism remains unclear.Aim To explore the relationship between CD83 and CD4+ T cell subsets and clarify the role of CD83 in the pathogenesis of ITP.Methods RT-qPCR and Flow cytometry were used to illustrate CD83 expression. The downregulation and overexpression of DC-CD83 were co-cultured with CD4+ T cells to detect cell proliferation, co-cultured supernatant cytokines and Tregs expression.Results The results indicate that the ITP patients showed higher expression of CD83 than the healthy controls. The proliferation of CD4+ T cells was inhibited by downregulation of DCs-CD83 but promoted by overexpression of DCs-CD83. siRNA-CD83 inhibited proinflammatory IFN-γ and IL-17 secretion while raising TGF-β, IL-10 concentrations. Overexpression of DCs-CD83 promoted Tregs expression.Conclusion The Th1/Th2 and Th17/Tregs polarization were reversed via interfering DCs with siRNA-CD83. CD83 plays an important role in ITP pathogenesis, suggesting novel treatment for ITP patients.

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