Dual-wavelength, nanosecond, miniature Raman laser enables efficient photoacoustic differentiation of water and lipid
Hanjie Wang,
Lin Zhao,
Huiyue You,
Huiling Wu,
Qingliang Zhao,
Xin Dong,
Shengchuang Bai,
Hongsen He,
Jun Dong
Affiliations
Hanjie Wang
Laboratory of Laser and Applied Photonics (LLAP), Department of Electronic Engineering, Xiamen University, Xiamen, China
Lin Zhao
Laboratory of Laser and Applied Photonics (LLAP), Department of Electronic Engineering, Xiamen University, Xiamen, China
Huiyue You
Laboratory of Laser and Applied Photonics (LLAP), Department of Electronic Engineering, Xiamen University, Xiamen, China
Huiling Wu
State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Center for Molecular Imaging and Translational Medicine, School of Public Health, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen 361102, China
Qingliang Zhao
State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Center for Molecular Imaging and Translational Medicine, School of Public Health, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen 361102, China
Xin Dong
Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, China
Shengchuang Bai
Laboratory of Infrared Material and Devices, The Research Institute of Advanced Technologies, College of Information Science and Engineering, Ningbo University, Ningbo 315211, China
Hongsen He
Laboratory of Laser and Applied Photonics (LLAP), Department of Electronic Engineering, Xiamen University, Xiamen, China
Jun Dong
Laboratory of Laser and Applied Photonics (LLAP), Department of Electronic Engineering, Xiamen University, Xiamen, China
Functional photoacoustic microscopy (PAM) requires laser sources with multiple wavelengths targeting abundant substances, where lipid and water are important components of living organisms. Here, we propose to use a single compact dual-wavelength passively Q-switched solid-state laser as the excitation source to directly achieve PA differentiation of water and lipid simultaneously. The main contribution of our work is to use the excitation difference under 1064- and 1176-nm lasers for mapping water and lipid in PAM, respectively. Meanwhile, the miniature structure (cavity size: ∼10 × 10 × 5.5 mm3) of the laser source is not only promising for portable applications but also benefits the PA-desired nanosecond (<2 ns) laser pulse establishment. Our technique is confirmed by efficient PA imaging of water and lipid in biological tissues at high spatial resolution and improved sensitivity. This laser provides a novel and low-cost imaging source for PAM to track changes in water and lipid distribution.
