Dataset of ileum bacterial diversity in mice after heart failure due to pressure overload
Martina E. Spehlmann,
Dhiraj P. Dhotre,
Nesrin Schmiedel,
Nikita Chavan,
Corinna Bang,
Ashraf Y. Rangrez
Affiliations
Martina E. Spehlmann
Department of Internal Medicine III, Cardiology, Angiology and Intensive Care Medicine, University Hospital of Schleswig-Holstein, Rosalind-Franklin Str. 12, Kiel 24105, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck, Germany
Dhiraj P. Dhotre
National Centre for Microbial Resource, National Centre for Cell Science, Pune 411021, India
Nesrin Schmiedel
Department of Internal Medicine III, Cardiology, Angiology and Intensive Care Medicine, University Hospital of Schleswig-Holstein, Rosalind-Franklin Str. 12, Kiel 24105, Germany; DZHK (German Centre for Cardiovascular Research), Partner Site Hamburg/Kiel/Lübeck, Germany
Nikita Chavan
National Centre for Microbial Resource, National Centre for Cell Science, Pune 411021, India
Corinna Bang
Institute of Clinical Molecular Biology, Christian-Albrechts-University Kiel, Rosalind-Franklin-Strasse 12, Kiel 24105, Germany
Ashraf Y. Rangrez
Department of Internal Medicine III, Cardiology, Angiology and Intensive Care Medicine, University Hospital of Schleswig-Holstein, Rosalind-Franklin Str. 12, Kiel 24105, Germany; Department of Internal Medicine III, University of Heidelberg, Im Neuenheimer Feld 410, Heidelberg 69120, Germany; DZHK, Partner Site, Heidelberg, Mannheim, Germany; Corresponding author at: Department of Internal Medicine III, University of Heidelberg, Im Neuenheimer Feld 410, Heidelberg 69120, Germany.
We recently reported the correlation of gut bacterial diversity with heart failure using a mouse model of heart failure due to pressure overload induced by transverse aortic constriction (TAC). We found that gut the bacterial diversity is significantly altered and is directly correlated to the severity of heart failure (Heart Failure Severity Closely Correlates with Intestinal Dysbiosis and Subsequent Metabolomic Alterations (Spehlmann, 2022). In addition, stool samples that were collected for the gut microbial diversity analysis, we dissected ileum from the mice after 42 days of TAC. The total DNA was extracted to identify the bacterial diversity resided in ileum using 16S rRNA gene amplicon shotgun sequencing and downstream bioinformatics analysis to determine if it is correlated to the heart failure.
