Effects of Various Explants and Hormone Combinations on in vitro Regeneration in Cucumber

Abstract

Effects of various explants and hormone combinations on in vitro regeneration in cucumber (Cucumis sativus) were studied using Jinyou No. 1. The results showed that explant types had a greater impact on there generation rate and number of regenerated buds in cucumber. When using 1–2-d-old sterilized cotyledons as explants, Murashige and Skoog (MS) supplemented with 6-benzylaminopurine (6-BA; 3.0 mg L-1), kinetin (KT; 1.0 mg L-1), abscisic acid (ABA; 1.0 mg L-1), and AgNO3 (2.0 mg L-1) was the best bud induction medium, with an adventitious bud regeneration rate of 73.3%, and 1.00 regenerated bud per explant. When using 5–6- d-old sterilized cotyledonary nodes as explants, MS supplemented with 6-BA (2.0 mg L-1) and AgNO3 (2.0 mg L-1) was the best, with an adventitious bud regeneration rate of 90.70% and 4.38 regenerated buds perexplant. In this study, cotyledonary nodes were more suitable than cotyledons for the establishment of the in vitro regeneration system in cucumber, and MS containing 6-BA (2.0 mg L-1) was the best bud induction medium for cotyledonary nodes, which may have valuable applications for an efficient in vitro regeneration protocol.