IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication
Junhao Chen,
Ruihua Zhang,
Jingjing Lan,
Shaoli Lin,
Pengfei Li,
Jiming Gao,
Yu Wang,
Zhi-Jing Xie,
Fu-Chang Li,
Shi-Jin Jiang
Affiliations
Junhao Chen
College of Veterinary Medicine, Shandong Agricultural University, Taian 271000, Shandong, China
Ruihua Zhang
College of Veterinary Medicine, Shandong Agricultural University, Taian 271000, Shandong, China
Jingjing Lan
College of Veterinary Medicine, Shandong Agricultural University, Taian 271000, Shandong, China
Shaoli Lin
Molecular Virology Laboratory, VA-MD College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA
Pengfei Li
College of Veterinary Medicine, Shandong Agricultural University, Taian 271000, Shandong, China
Jiming Gao
Department of Basic Medical Sciences, Taishan Medical College, Taian 271000, Shandong, China
Yu Wang
Department of Basic Medical Sciences, Taishan Medical College, Taian 271000, Shandong, China
Zhi-Jing Xie
College of Veterinary Medicine, Shandong Agricultural University, Taian 271000, Shandong, China
Fu-Chang Li
College of Animal Science and Technology, Shandong Agricultural University, Taian 271000, Shandong, China
Shi-Jin Jiang
College of Veterinary Medicine, Shandong Agricultural University, Taian 271000, Shandong, China
As a disease characterized by severe liver necrosis and hemorrhage, duck viral hepatitis (DVH) is mainly caused by duck hepatitis A virus (DHAV). The positive-strand RNA genome of DHAV type 1 (DHAV-1) contains an internal ribosome entry site (IRES) element within the 5′ untranslated region (UTR), structured sequence elements within the 3′ UTR, and a poly(A) tail at the 3′ terminus. In this study, we first examined that insulin-like growth factor-2 mRNA-binding protein-1 (IGF2BP1) specifically interacted with the DHAV-1 3′ UTR by RNA pull-down assay. The interaction between IGF2BP1 and DHAV-1 3′ UTR strongly enhanced IRES-mediated translation efficiency but failed to regulate DHAV-1 replication in a duck embryo epithelial (DEE) cell line. The viral propagation of DHAV-1 strongly enhanced IGF2BP1 expression level, and viral protein accumulation was identified as the key point to this increment. Collectively, our data demonstrated the positive role of IGF2BP1 in DHAV-1 viral proteins translation and provided data support for the replication mechanism of DHAV-1.
