Development of a Deep Learning Model for the Analysis of Dorsal Root Ganglion Chromatolysis in Rat Spinal Stenosis

Abstract

Meihui Li,1,2 Haiyan Zheng,3 Jae Chul Koh,4 Ghee Young Choe,5,6 Eun Joo Choi,1,2 Francis Sahngun Nahm,1,2 Pyung Bok Lee1,2 1Department of Anesthesiology and Pain Medicine, Seoul National University College of Medicine, Seoul, Korea; 2Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam, Korea; 3Department of Anesthesiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of China; 4Department of Anesthesiology and Pain Medicine, Korea University College of Medicine, Seoul, Korea; 5Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea; 6Department of Pathology, Seoul National University College of Medicine, Seoul, KoreaCorrespondence: Pyung Bok Lee, Email [email protected]: To create a deep learning (DL) model that can accurately detect and classify three distinct types of rat dorsal root ganglion neurons: normal, segmental chromatolysis, and central chromatolysis. The DL model has the potential to improve the efficiency and precision of neuron classification in research related to spinal injuries and diseases.Methods: H&E slide images were divided into an internal training set (80%) and a test set (20%). The training dataset was labeled by two pathologists using pre-defined grades. Using this dataset, a two-component DL model was developed with the first component being a convolutional neural network (CNN) that was trained to detect the region of interest (ROI) and the second component being another CNN used for classification.Results: A total of 240 lumbar dorsal root ganglion (DRG) pathology slide images from rats were analyzed. The internal testing results showed an accuracy of 93.13%, and the external dataset testing demonstrated an accuracy of 93.44%.Conclusion: The DL model demonstrated a level of agreement comparable to that of pathologists in detecting and classifying normal and segmental chromatolysis neurons, although its agreement was slightly lower for central chromatolysis neurons. Significance: DL in improving the accuracy and efficiency of pathological analysis suggests that it may have a role in enhancing medical decision-making.Keywords: deep learning, dorsal root ganglion, chromatolysis, automated detection and spinal stenosis

Keywords

• deep learning
• dorsal root ganglion
• chromatolysis
• automated detection and spinal stenosis.