The Effect of Mesenchymal Stem Cells Derived-Conditioned Media in Combination with Oral Anti-Androgenic Drugs on Male Pattern Baldness: An Animal Study
Majid Kamali-Dolat Abadi,
Gholamhossein Yousefi,
Farzaneh Dehghani,
Ali Alizadeh,
Abolfazl Jangholi,
Mohammad Amin Moadab,
Maryam Naseh,
Shima Parsa,
Golara Nasiri,
Negar Azarpira,
Mehdi Dianatpour
Affiliations
Majid Kamali-Dolat Abadi
Department of Tissue Engineering and Applied Cell Sciences, School of Advance Medical Science and Technology, Shiraz University of Medical Sciences, Shiraz, Iran
Gholamhossein Yousefi
Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Farzaneh Dehghani
Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Ali Alizadeh
Department of Tissue Engineering and applied cell science, School of Advanced Medical Sciences & Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
Abolfazl Jangholi
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
Mohammad Amin Moadab
Department of Biology, Zand Institute of Higher Education, Shiraz, Iran
Maryam Naseh
Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Shima Parsa
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Golara Nasiri
Department of Tissue Engineering and Cell Therapy, School of Advanced Technologies in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Negar Azarpira
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Mehdi Dianatpour
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Objective: Androgenetic alopecia (AGA) is a prevalent form of hair loss, mainly caused by follicular sensitivity toandrogens. Despite developing different anti-androgen treatment options, the success rate of these treatments hasbeen limited. Using animal models, this study evaluated the therapeutic effects of umbilical cord (UC) stem cellconditioned media (CM) combined with oral anti-androgens for hair regeneration.Materials and Methods: In this experimental study, Poloxamer 407 (P407) was used as a drug carrier forsubcutaneous testosterone injection. AGA models were treated with oral finasteride, oral flutamide, and CMinjections. Samples were thoroughly evaluated and compared using histological, stereological, and molecularanalyses.Results: Injecting CM-loaded hydrogel alone or combined with oral intake of anti-androgens improved hair regeneration.These treatments could promote hair growth by inducing hair follicles in the anagen stage and shortening the telogenand catagen phases. Furthermore, the combination treatment led to an upregulation of hair induction gene expressionwith a downregulation of inflammation genes.Conclusion: Through a reduction in inflammation, injection of CM-loaded hydrogel alone or combined with oral intakeof anti-androgens induces the hair cell cycle with regeneration in damaged follicles. Hence, this could be a promisingtherapeutic method for AGA patients.
