Veterinary Medicine and Science (Mar 2021)

A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

  • Saeed El‐Ashram,
  • Yu Zhang,
  • Yongsheng Ji,
  • Dina Salama,
  • Kun Mei,
  • Li Zhili,
  • Huang Shujian,
  • Haoji Zhang,
  • Shawky M. Aboelhadid,
  • Reem A. Alajmi,
  • Dina M. Metwally,
  • Manal F. El‐Khadragy,
  • Billy M. Hargis,
  • Guillermo Tellez‐Isaias,
  • Beniamino T. Cenci‐Goga,
  • Musafiri Karama,
  • Munyaradzi C. Marufu,
  • Fathi Abouhajer,
  • Gamal Ali Abdelhafez Hamady,
  • Abeer El Wakil,
  • Ibrahim Al Nasr,
  • Xun Suo

Journal volume & issue
Vol. 7, no. 2
pp. 357 – 361


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Abstract This study describes a simple method for the large‐scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5 min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure T. gondii tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive.