Nature Communications (Jul 2019)

Structure-based mechanism for activation of the AAA+ GTPase McrB by the endonuclease McrC

  • Neha Nirwan,
  • Yuzuru Itoh,
  • Pratima Singh,
  • Sutirtha Bandyopadhyay,
  • Kutti R. Vinothkumar,
  • Alexey Amunts,
  • Kayarat Saikrishnan

DOI
https://doi.org/10.1038/s41467-019-11084-1
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 9

Abstract

Read online

McrBC is a bacterial antiphage defense system that cleaves methylated DNA and is composed of the AAA+ GTPase motor McrB and the endonuclease McrC. Here, the authors present the cryo-EM structure of E. coli McrBC that reveals how McrC inserts a stalk-like structure into the pore of the ring-shaped McrB hexamer and discuss mechanistic implications.