Mechanical, Histological, and Scanning Electron Microscopy Study of the Effect of Mixed-Acid and Heat Treatment on Additive-Manufactured Titanium Plates on Bonding to the Bone Surface

Naoko Imagawa; Kazuya Inoue; Keisuke Matsumoto; Ayako Ochi; Michi Omori; Kayoko Yamamoto; Yoichiro Nakajima; Nahoko Kato-Kogoe; Hiroyuki Nakano; Tomiharu Matsushita; Seiji Yamaguchi; Phuc Thi Minh Le; Shinpei Maruyama; Takaaki Ueno

doi:10.3390/ma13225104

Materials (Nov 2020)

Mechanical, Histological, and Scanning Electron Microscopy Study of the Effect of Mixed-Acid and Heat Treatment on Additive-Manufactured Titanium Plates on Bonding to the Bone Surface

Naoko Imagawa,
Kazuya Inoue,
Keisuke Matsumoto,
Ayako Ochi,
Michi Omori,
Kayoko Yamamoto,
Yoichiro Nakajima,
Nahoko Kato-Kogoe,
Hiroyuki Nakano,
Tomiharu Matsushita,
Seiji Yamaguchi,
Phuc Thi Minh Le,
Shinpei Maruyama,
Takaaki Ueno

Affiliations

Naoko Imagawa: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Kazuya Inoue: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Keisuke Matsumoto: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Ayako Ochi: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Michi Omori: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Kayoko Yamamoto: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Yoichiro Nakajima: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Nahoko Kato-Kogoe: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Hiroyuki Nakano: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan
Tomiharu Matsushita: Department of Biomedical Science, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan
Seiji Yamaguchi: Department of Biomedical Science, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan
Phuc Thi Minh Le: Department of Biomedical Science, College of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan
Shinpei Maruyama: Osaka Yakin Kogyo Co., Ltd., 4-4-28, Zuiko, Yodogawa-ku, Osaka 533-0005, Japan
Takaaki Ueno: Division of Medicine for Function and Morphology of Sensor Organs, Department of Dentistry and Oral Surgery, Faculty of Medicine, Osaka Medical College, 2-7 Daigakumachi, Takatsuki, Osaka 569-8686, Japan

DOI: https://doi.org/10.3390/ma13225104
Journal volume & issue: Vol. 13, no. 22
p. 5104

Abstract

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The additive manufacturing (AM) technique has attracted attention as one of the fully customizable medical material technologies. In addition, the development of new surface treatments has been investigated to improve the osteogenic ability of the AM titanium (Ti) plate. The purpose of this study was to evaluate the osteogenic activity of the AM Ti with mixed-acid and heat (MAH) treatment. Fully customized AM Ti plates were created with a curvature suitable for rat calvarial bone, and they were examined in a group implanted with the MAH-treated Ti in comparison with the untreated (UN) group. The AM Ti plates were fixed to the surface of rat calvarial bone, followed by extraction of the calvarial bone 1, 4, 8, and 12 weeks after implantation. The bonding between the bone and Ti was evaluated mechanically. In addition, AM Ti plates removed from the bone were examined histologically by electron microscopy and Villanueva–Goldner stain. The mechanical evaluation showed significantly stronger bone-bonding in the MAH group than in the UN group. In addition, active bone formation was seen histologically in the MAH group. Therefore, these findings indicate that MAH resulted in rapid and strong bonding between cortical bone and Ti.

Published in Materials

ISSN: 1996-1944 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering; Technology: Engineering (General). Civil engineering (General); Science: Natural history (General): Microscopy; Science: Physics: Descriptive and experimental mechanics
Website: http://www.mdpi.com/journal/materials/

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