Towards Electrochemical Sensor Based on Molecularly Imprinted Polypyrrole for the Detection of Bacteria—<i>Listeria monocytogenes</i>

Viktorija Liustrovaite; Maksym Pogorielov; Raimonda Boguzaite; Vilma Ratautaite; Almira Ramanaviciene; Greta Pilvenyte; Viktoriia Holubnycha; Viktoriia Korniienko; Kateryna Diedkova; Roman Viter; Arunas Ramanavicius

doi:10.3390/polym15071597

Polymers (Mar 2023)

Towards Electrochemical Sensor Based on Molecularly Imprinted Polypyrrole for the Detection of Bacteria—<i>Listeria monocytogenes</i>

Viktorija Liustrovaite,
Maksym Pogorielov,
Raimonda Boguzaite,
Vilma Ratautaite,
Almira Ramanaviciene,
Greta Pilvenyte,
Viktoriia Holubnycha,
Viktoriia Korniienko,
Kateryna Diedkova,
Roman Viter,
Arunas Ramanavicius

Affiliations

Viktorija Liustrovaite: Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania
Maksym Pogorielov: Biomedical Research Centre, Sumy State University, R-Korsakova Street, 40007 Sumy, Ukraine
Raimonda Boguzaite: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology, Saulėtekio Av. 3, LT-10257 Vilnius, Lithuania
Vilma Ratautaite: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology, Saulėtekio Av. 3, LT-10257 Vilnius, Lithuania
Almira Ramanaviciene: NanoTechnas-Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania
Greta Pilvenyte: Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology, Saulėtekio Av. 3, LT-10257 Vilnius, Lithuania
Viktoriia Holubnycha: Biomedical Research Centre, Sumy State University, R-Korsakova Street, 40007 Sumy, Ukraine
Viktoriia Korniienko: Biomedical Research Centre, Sumy State University, R-Korsakova Street, 40007 Sumy, Ukraine
Kateryna Diedkova: Biomedical Research Centre, Sumy State University, R-Korsakova Street, 40007 Sumy, Ukraine
Roman Viter: Institute of Atomic Physics and Spectroscopy, University of Latvia, Jelgavas iela 3, LV-1004 Riga, Latvia
Arunas Ramanavicius: Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania

DOI: https://doi.org/10.3390/polym15071597
Journal volume & issue: Vol. 15, no. 7
p. 1597

Abstract

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Detecting bacteria—Listeria monocytogenes—is an essential healthcare and food industry issue. The objective of the current study was to apply platinum (Pt) and screen-printed carbon (SPCE) electrodes modified by molecularly imprinted polymer (MIP) in the design of an electrochemical sensor for the detection of Listeria monocytogenes. A sequence of potential pulses was used to perform the electrochemical deposition of the non-imprinted polypyrrole (NIP-Ppy) layer and Listeria monocytogenes-imprinted polypyrrole (MIP-Ppy) layer over SPCE and Pt electrodes. The bacteria were removed by incubating Ppy-modified electrodes in different extraction solutions (sulphuric acid, acetic acid, L-lysine, and trypsin) to determine the most efficient solution for extraction and to obtain a more sensitive and repeatable design of the sensor. The performance of MIP-Ppy- and NIP-Ppy-modified electrodes was evaluated by pulsed amperometric detection (PAD). According to the results of this research, it can be assumed that the most effective MIP-Ppy/SPCE sensor can be designed by removing bacteria with the proteolytic enzyme trypsin. The LOD and LOQ of the MIP-Ppy/SPCE were 70 CFU/mL and 210 CFU/mL, respectively, with a linear range from 300 to 6700 CFU/mL.

Published in Polymers

ISSN: 2073-4360 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/polymers/

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