A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

Xiaoting Wu; Xiaoting Wu; Xiangyu Yang; Lulu Song; Yang Wang; Yang Wang; Yamin Li; Yamin Li; Yuanyuan Liu; Yuanyuan Liu; Xiaowei Yang; Yijun Wang; Yijun Wang; Yijun Wang; Weihua Pei; Weihua Pei; Weihua Pei; Weidong Li

doi:10.3389/fnint.2021.682019

Frontiers in Integrative Neuroscience (Aug 2021)

A Modified Miniscope System for Simultaneous Electrophysiology and Calcium Imaging in vivo

Xiaoting Wu,
Xiaoting Wu,
Xiangyu Yang,
Lulu Song,
Yang Wang,
Yang Wang,
Yamin Li,
Yamin Li,
Yuanyuan Liu,
Yuanyuan Liu,
Xiaowei Yang,
Yijun Wang,
Yijun Wang,
Yijun Wang,
Weihua Pei,
Weihua Pei,
Weihua Pei,
Weidong Li

Affiliations

Xiaoting Wu: The State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China
Xiaoting Wu: University of Chinese Academy of Sciences, Beijing, China
Xiangyu Yang: Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China
Lulu Song: Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China
Yang Wang: The State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China
Yang Wang: University of Chinese Academy of Sciences, Beijing, China
Yamin Li: The State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China
Yamin Li: University of Chinese Academy of Sciences, Beijing, China
Yuanyuan Liu: The State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China
Yuanyuan Liu: University of Chinese Academy of Sciences, Beijing, China
Xiaowei Yang: The State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China
Yijun Wang: The State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China
Yijun Wang: University of Chinese Academy of Sciences, Beijing, China
Yijun Wang: CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai, China
Weihua Pei: The State Key Laboratory on Integrated Optoelectronics, Institute of Semiconductors, Chinese Academy of Sciences, Beijing, China
Weihua Pei: University of Chinese Academy of Sciences, Beijing, China
Weihua Pei: CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai, China
Weidong Li: Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China

DOI: https://doi.org/10.3389/fnint.2021.682019
Journal volume & issue: Vol. 15

Abstract

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The miniscope system is one of the calcium (Ca2+) imaging tools with small size and lightweight and can realize the deep-brain Ca2+ imaging not confined to the cerebral cortex. Combining Ca2+ imaging and electrophysiology recording has been an efficient method for extracting high temporal-spatial resolution signals in the brain. In this study, a particular electrode probe was developed and assembled on the imaging lens to modify the miniscope system. The electrode probe can be tightly integrated into the lens of the miniscope without increasing the volume, weight, and implantation complexity. In vivo tests verified that the proposed modified system has realized the simultaneous recording of Ca2+ signals and local field potential (LFP) signal in the hippocampus CA1 region of an adult mouse.

Published in Frontiers in Integrative Neuroscience

ISSN: 1662-5145 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry: Neurology. Diseases of the nervous system
Website: https://www.frontiersin.org/journals/integrative-neuroscience

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