Antiulcer Activity of Anthraquinone–Flavonoid Complex of <i>Rumex tianschanicus</i> Losinsk
Gulnaz A. Seitimova,
Aksholpan K. Shokan,
Tatyana G. Tolstikova,
Nataliya A. Zhukova,
Dmitriy Yu. Korulkin,
Nataliya O. Kudrina,
Yuliya A. Litvinenko,
Nataliya D. Meduntseva,
Nina V. Terletskaya,
Timur E. Kulmanov
Affiliations
Gulnaz A. Seitimova
Faculty of Biology and Biotechnology and Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Al-Farabi Av., 71, Almaty 050040, Kazakhstan
Aksholpan K. Shokan
Faculty of Biology and Biotechnology and Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Al-Farabi Av., 71, Almaty 050040, Kazakhstan
Tatyana G. Tolstikova
N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of Russian Academy of Science, 630090 Novosibirsk, Russia
Nataliya A. Zhukova
N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of Russian Academy of Science, 630090 Novosibirsk, Russia
Dmitriy Yu. Korulkin
Faculty of Biology and Biotechnology and Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Al-Farabi Av., 71, Almaty 050040, Kazakhstan
Nataliya O. Kudrina
Faculty of Biology and Biotechnology and Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Al-Farabi Av., 71, Almaty 050040, Kazakhstan
Yuliya A. Litvinenko
Faculty of Biology and Biotechnology and Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Al-Farabi Av., 71, Almaty 050040, Kazakhstan
Nataliya D. Meduntseva
Faculty of Biology and Biotechnology and Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Al-Farabi Av., 71, Almaty 050040, Kazakhstan
Nina V. Terletskaya
Faculty of Biology and Biotechnology and Faculty of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Al-Farabi Av., 71, Almaty 050040, Kazakhstan
Timur E. Kulmanov
Institute of Genetic and Physiology, Al-Farabi Av., 93, Almaty 050040, Kazakhstan
The composition of an ethanol extract from the roots of Rumex tianschanicus Losinsk of the Trans-Ili Alatau wild flora was studied in order to determine its antiulcer activity. The phytochemical composition of the anthraquinone–flavonoid complex from (AFC) R. tianschanicus revealed the presence of numerous polyphenolic compounds, the most abundant of which are anthraquinones (1.77%), flavonoids (6.95%), and tannins (13.39%). The use of column chromatography (CC) and thin-layer chromatography (TLC) in conjunction with UV, IR, NMR spectroscopy, and mass spectrometry data allowed the researchers to isolate and identify the major components of the anthraquinone–flavonoid complex’s polyphenol fraction: physcion, chrysophanol, emodin, isorhamnetin, quercetin, and myricetin. The gastroprotective effect of the polyphenolic fraction of the anthraquinone–flavonoid complex (AFC) of R. tianschanicus roots was examined in an experimental model of rat gastric ulcer induced by indomethacin. The preventive and therapeutic effect of the anthraquinone–flavonoid complex at a dose of 100 mg/kg was analyzed using intragastric administration per day for 1 to 10 days, followed by a histological examination of stomach tissues. It has been demonstrated that prophylactic and prolonged use of the AFC R. tianschanicus in laboratory animals resulted in significantly less pronounced hemodynamic and desquamative changes in the epithelium of gastric tissues. The acquired results thus offer fresh insight into the anthraquinone and flavonoid metabolite component composition of R. tianschanicus roots, and they imply that the examined extract can be used to develop herbal medicines with antiulcer activity.
