Measuring the antibiotics and pesticides in honeys by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)(超高效液相色谱-串联质谱法测定蜂蜜中抗生素和农药浓度)

WANGJianmei(汪建妹); YANGHua(杨华); ZENGYinhuan(曾银欢); WULiqin(吴俐勤); LIRui(李锐); YUANYuwei(袁玉伟); QIANMingrong(钱鸣蓉)

doi:10.3785/j.issn.1008-9497.2018.03.010

Zhejiang Daxue xuebao. Lixue ban (May 2018)

Measuring the antibiotics and pesticides in honeys by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)(超高效液相色谱-串联质谱法测定蜂蜜中抗生素和农药浓度)

WANGJianmei(汪建妹),
YANGHua(杨华),
ZENGYinhuan(曾银欢),
WULiqin(吴俐勤),
LIRui(李锐),
YUANYuwei(袁玉伟),
QIANMingrong(钱鸣蓉)

Affiliations

WANGJianmei(汪建妹): ORCiD; Institute of Quality Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310014, China(浙江农业科学院农产品质量标准研究所，浙江杭州 310014)
YANGHua(杨华): Institute of Quality Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310014, China(浙江农业科学院农产品质量标准研究所，浙江杭州 310014)
ZENGYinhuan(曾银欢): Institute of Quality Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310014, China(浙江农业科学院农产品质量标准研究所，浙江杭州 310014)
WULiqin(吴俐勤): Institute of Quality Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310014, China(浙江农业科学院农产品质量标准研究所，浙江杭州 310014)
LIRui(李锐): Institute of Quality Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310014, China(浙江农业科学院农产品质量标准研究所，浙江杭州 310014)
YUANYuwei(袁玉伟): Institute of Quality Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310014, China(浙江农业科学院农产品质量标准研究所，浙江杭州 310014)
QIANMingrong(钱鸣蓉): ORCiD; Institute of Quality Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310014, China(浙江农业科学院农产品质量标准研究所，浙江杭州 310014)

DOI: https://doi.org/10.3785/j.issn.1008-9497.2018.03.010
Journal volume & issue: Vol. 45, no. 3
pp. 330 – 342

Abstract

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基于快速有效的基质净化方法，结合超高效液相色谱-串联质谱技术，建立了测定蜂蜜中39种抗生素及25 种农药的检测方法.蜂蜜样品经乙腈＋水（v/v=8：2）提取，用PRiME HLB净化，超高效液相色谱-串联质谱(ultra-performance liquid chromatography tandem mass spectrometry,UPLC-MS/MS)分析测定，通过多反应监测模式(MRM)分析目标化合物，并进行基质标准曲线定量.在测定浓度范围内呈良好线性关系，相关系数大于0.99；农兽药的检出限为0.02〜3.8 µg · kg-1，定量限为0.1〜11.5 µg · kg-1；在3档浓度的添加回收实验中，39种抗生素的回收率在72%〜119%内，25种农药的回收率在72%〜115%内，相对标准偏差均小于15%.随机抽取浙江市场上在售的52份蜂蜜样进行检测分析和膳食暴露评估，共检出罗红霉素、诺氟沙星和环丙沙星3种抗生素，检出率分别为2.6%，8.6%和3.9%，浓度分别为59.6〜214.4，4.7〜1 486.3和5.9〜215.1 µg · kg-1.膳食暴露评估结果表明，蜂蜜中抗生素残留对10岁以下组群的风险高于其他组群、女性消费者高于男性消费者；％ADI<100，说明膳食暴露评估风险水平尚可接受.因在计算中存在诸多不确定性因素，对组群的风险评估尚需进一步分析.

Published in Zhejiang Daxue xuebao. Lixue ban

ISSN: 1008-9497 (Print)
Publisher: Zhejiang University Press
Country of publisher: China
LCC subjects: Science: Mathematics: Instruments and machines: Electronic computers. Computer science; Science: Physics
Website: https://www.zjujournals.com/sci/EN/1008-9497/home.shtml

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