RNase III CLASH in MRSA uncovers sRNA regulatory networks coupling metabolism to toxin expression

Stuart W. McKellar; Ivayla Ivanova; Pedro Arede; Rachel L. Zapf; Noémie Mercier; Liang-Cui Chu; Daniel G. Mediati; Amy C. Pickering; Paul Briaud; Robert G. Foster; Grzegorz Kudla; J. Ross Fitzgerald; Isabelle Caldelari; Ronan K. Carroll; Jai J. Tree; Sander Granneman

doi:10.1038/s41467-022-31173-y

Nature Communications (Jun 2022)

RNase III CLASH in MRSA uncovers sRNA regulatory networks coupling metabolism to toxin expression

Stuart W. McKellar,
Ivayla Ivanova,
Pedro Arede,
Rachel L. Zapf,
Noémie Mercier,
Liang-Cui Chu,
Daniel G. Mediati,
Amy C. Pickering,
Paul Briaud,
Robert G. Foster,
Grzegorz Kudla,
J. Ross Fitzgerald,
Isabelle Caldelari,
Ronan K. Carroll,
Jai J. Tree,
Sander Granneman

Affiliations

Stuart W. McKellar: Centre for Synthetic and Systems Biology, University of Edinburgh
Ivayla Ivanova: Centre for Synthetic and Systems Biology, University of Edinburgh
Pedro Arede: Centre for Synthetic and Systems Biology, University of Edinburgh
Rachel L. Zapf: Department of Biological Sciences, Ohio University
Noémie Mercier: Université de Strasbourg, CNRS, Architecture et Réactivité de l’ARN, UPR9002
Liang-Cui Chu: Centre for Synthetic and Systems Biology, University of Edinburgh
Daniel G. Mediati: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Amy C. Pickering: The Roslin Institute and Edinburgh Infectious Diseases, University of Edinburgh, Easter Bush Campus
Paul Briaud: Department of Biological Sciences, Ohio University
Robert G. Foster: MRC Human Genetics Unit, University of Edinburgh
Grzegorz Kudla: MRC Human Genetics Unit, University of Edinburgh
J. Ross Fitzgerald: The Roslin Institute and Edinburgh Infectious Diseases, University of Edinburgh, Easter Bush Campus
Isabelle Caldelari: Université de Strasbourg, CNRS, Architecture et Réactivité de l’ARN, UPR9002
Ronan K. Carroll: Department of Biological Sciences, Ohio University
Jai J. Tree: School of Biotechnology and Biomolecular Sciences, University of New South Wales
Sander Granneman: Centre for Synthetic and Systems Biology, University of Edinburgh

DOI: https://doi.org/10.1038/s41467-022-31173-y
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 20

Abstract

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Regulatory small RNA (sRNA) interact with mRNAs to regulate their stability, transcription, and translation via diverse mechanisms. Here, McKellar et al. apply RNase IIICLASH of multi-drug resistant Staphylococcus aureus under different culture conditions to link the network of RNA-RNA interactions to environmental conditions and find that the production of small membrane-permeabilizing toxins is strongly regulated by sRNAs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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