Efficient in planta production of amidated antimicrobial peptides that are active against drug-resistant ESKAPE pathogens

Shahid Chaudhary; Zahir Ali; Muhammad Tehseen; Evan F. Haney; Aarón Pantoja-Angles; Salwa Alshehri; Tiannyu Wang; Gerard J. Clancy; Maya Ayach; Charlotte Hauser; Pei-Ying Hong; Samir M. Hamdan; Robert E. W. Hancock; Magdy Mahfouz

doi:10.1038/s41467-023-37003-z

Nature Communications (Mar 2023)

Efficient in planta production of amidated antimicrobial peptides that are active against drug-resistant ESKAPE pathogens

Shahid Chaudhary,
Zahir Ali,
Muhammad Tehseen,
Evan F. Haney,
Aarón Pantoja-Angles,
Salwa Alshehri,
Tiannyu Wang,
Gerard J. Clancy,
Maya Ayach,
Charlotte Hauser,
Pei-Ying Hong,
Samir M. Hamdan,
Robert E. W. Hancock,
Magdy Mahfouz

Affiliations

Shahid Chaudhary: Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology (KAUST)
Zahir Ali: Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology (KAUST)
Muhammad Tehseen: Laboratory of DNA Replication and Recombination, Division of Biological Sciences and Engineering, King Abdullah University of Science and Technology (KAUST)
Evan F. Haney: Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia
Aarón Pantoja-Angles: Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology (KAUST)
Salwa Alshehri: Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST)
Tiannyu Wang: Water Desalination and Reuse Center, Division of Biological Sciences and Engineering, King Abdullah University of Science and Technology (KAUST)
Gerard J. Clancy: Analytical Chemistry Core Laboratory, King Abdullah University of Science and Technology (KAUST)
Maya Ayach: Imaging & Characterization Core Laboratory, King Abdullah University of Science and Technology (KAUST)
Charlotte Hauser: Laboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology (KAUST)
Pei-Ying Hong: Water Desalination and Reuse Center, Division of Biological Sciences and Engineering, King Abdullah University of Science and Technology (KAUST)
Samir M. Hamdan: Laboratory of DNA Replication and Recombination, Division of Biological Sciences and Engineering, King Abdullah University of Science and Technology (KAUST)
Robert E. W. Hancock: Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia
Magdy Mahfouz: Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology (KAUST)

DOI: https://doi.org/10.1038/s41467-023-37003-z
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 16

Abstract

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Antimicrobial peptides (AMPs) are next-generation antibiotics that can be used to combat drugresistant pathogens. Here, the authors report efficient production of bioactive amidated AMPs by transient expression in Nicotiana benthamiana line expressing the mammalian enzyme peptidylglycine α-amidating mono-oxygenase.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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