Separation of Biological Entities from Human Blood by Using Magnetic Nanocomposites Obtained from Zeolite Precursors

Serena Esposito; Antonello Marocco; Gianfranco Dell’Agli; Barbara Bonelli; Franca Mannu; Paolo Allia; Paola Tiberto; Gabriele Barrera; Michele Pansini

doi:10.3390/molecules25081803

Molecules (Apr 2020)

Separation of Biological Entities from Human Blood by Using Magnetic Nanocomposites Obtained from Zeolite Precursors

Serena Esposito,
Antonello Marocco,
Gianfranco Dell’Agli,
Barbara Bonelli,
Franca Mannu,
Paolo Allia,
Paola Tiberto,
Gabriele Barrera,
Michele Pansini

Affiliations

Serena Esposito: Department of Applied Science and Technology and INSTM Unit of Torino-Politecnico, Corso Duca degli Abruzzi 24, Politecnico di Torino, 10129 Torino, Italy
Antonello Marocco: Department of Civil and Mechanical Engineering and INSTM Research Unit, Università degli Studi di Cassino e del Lazio Meridionale, Via G. Di Biasio 43, 03043 Cassino, FR, Italy
Gianfranco Dell’Agli: Department of Civil and Mechanical Engineering and INSTM Research Unit, Università degli Studi di Cassino e del Lazio Meridionale, Via G. Di Biasio 43, 03043 Cassino, FR, Italy
Barbara Bonelli: Department of Applied Science and Technology and INSTM Unit of Torino-Politecnico, Corso Duca degli Abruzzi 24, Politecnico di Torino, 10129 Torino, Italy
Franca Mannu: Nurex srl, Z.I. Predda Niedda, 07100 Sassari, Italy
Paolo Allia: Department of Applied Science and Technology and INSTM Unit of Torino-Politecnico, Corso Duca degli Abruzzi 24, Politecnico di Torino, 10129 Torino, Italy
Paola Tiberto: INRIM, Nanoscience and Materials Division, Strada delle Cacce 91, 10135 Torino, Italy
Gabriele Barrera: INRIM, Nanoscience and Materials Division, Strada delle Cacce 91, 10135 Torino, Italy
Michele Pansini: Department of Civil and Mechanical Engineering and INSTM Research Unit, Università degli Studi di Cassino e del Lazio Meridionale, Via G. Di Biasio 43, 03043 Cassino, FR, Italy

DOI: https://doi.org/10.3390/molecules25081803
Journal volume & issue: Vol. 25, no. 8
p. 1803

Abstract

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In this work, three novel magnetic metal–ceramic nanocomposites were obtained by thermally treating Fe-exchanged zeolites (either A or X) under reducing atmosphere at relatively mild temperatures (750–800 °C). The so-obtained materials were thoroughly characterized from the point of view of their physico-chemical properties and, then, used as magnetic adsorbents in the separation of the target gene factors V and RNASE and of the Staphylococcus aureus bacteria DNA from human blood. Such results were compared with those obtained by using a top ranking commercial separation system (namely, SiMAG-N-DNA by Chemicell). The results obtained by using the novel magnetic adsorbents were similar to (or even better than) those obtained by using the commercial system, both during manual and automated separations, provided that a proper protocol was adopted. Particularly, the novel magnetic adsorbents showed high sensitivity during tests performed with small volumes of blood. Finally, the feasible production of such magnetic adsorbents by an industrial process was envisaged as well.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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