Nature Communications (Aug 2020)

CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs

  • Matthew A. Coelho,
  • Etienne De Braekeleer,
  • Mike Firth,
  • Michal Bista,
  • Sebastian Lukasiak,
  • Maria Emanuela Cuomo,
  • Benjamin J. M. Taylor

DOI
https://doi.org/10.1038/s41467-020-17952-5
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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Off-target editing remains a concern for therapeutic applications of CRISPR-Cas9. Here the authors present CRISPR GUARD, which uses very short non-cleaving gRNAs to prevent editing at off-target sites.