Association of WWOX Gene Expression with Chronic Lymphocytic Leukemia

Abstract

Aim:The WW domain-containing oxidoreductase (WWOX) gene is located on chromosome 16q23.3-q24.1 and contains the common chromosomal fragile site, FRA16D. The WWOX gene encodes a Wwox tumor suppressor protein with a molecular weight of 46 kDa. Loss of heterozygosity (LOH) at the WWOX locus, promoter hypermethylation of the WWOX promoter, and consequently Wwox expression loss or reduction has been reported in a large fraction of many human cancers. Also, recent studies have shown that Wwox deficiency is associated with poor prognosis in various types of cancer. Clinical features and genetic anomalies of chronic lymphocytic leukemia (CLL) are well defined, but molecular details are still under investigation. WWOX expression levels could be a possible biomarker for CLL. As much as we know, there is no evidence for diagnostic and prognostic significance of Wwox in CLL. In our study, we aimed to define the expression levels of WWOX in CLL patients and also to analyze the WWOX expression in CLL patients with regard to their clinical characteristics.Materials and Methods:We performed this study in 40 CLL patients and 26 healthy controls. We analyzed the WWOX expression levels by using reverse transcriptase-quantitative PCR (RT-QPCR). Results: Our results showed that WWOX expression was significantly higher in CLL patients compared to healthy control group (P0.05).Results:Our results showed that WWOX expression was significantly higher in CLL patients compared to healthy control group (P0.05).Conclusion:Abnormal transcription variants of WWOX gene can be associated with abnormal protein isoforms and these isoforms can change the tumor suppressive effects of WWOX gene in CLL patients.

Keywords

• age-related macular degeneration; off-label drug
• vascular endothelial growth factor
• vegf