Effect of FTY720 inhibiting corneal neovascularition induced by sphingosine 1-phosphate

Fan Zhong; Xiao-Zhen Ding; Wei-Zhong Yang; Zong-Yin Gao; Xiao-He Lu

doi:10.3980/j.issn.1672-5123.2015.7.04

Guoji Yanke Zazhi (Jul 2015)

Effect of FTY720 inhibiting corneal neovascularition induced by sphingosine 1-phosphate

Fan Zhong,
Xiao-Zhen Ding,
Wei-Zhong Yang,
Zong-Yin Gao,
Xiao-He Lu

Affiliations

Fan Zhong: Department of Ophthalmology, the Affiliated Guangzhou First Municipal People's Hospital of Guangzhou Medical College, Guangzhou 510180, Guangdong Province, China
Xiao-Zhen Ding: Department of Ophthalmology, the Affiliated Guangzhou First Municipal People's Hospital of Guangzhou Medical College, Guangzhou 510180, Guangdong Province, China
Wei-Zhong Yang: Department of Ophthalmology, the Affiliated Guangzhou First Municipal People's Hospital of Guangzhou Medical College, Guangzhou 510180, Guangdong Province, China
Zong-Yin Gao: Department of Ophthalmology, the Affiliated Guangzhou First Municipal People's Hospital of Guangzhou Medical College, Guangzhou 510180, Guangdong Province, China
Xiao-He Lu: Department of Ophthalmology, the Affiliated Zhujiang Hospital of Southern Medical Univesity, Guangzhou 510180, Guangdong Province, China

DOI: https://doi.org/10.3980/j.issn.1672-5123.2015.7.04
Journal volume & issue: Vol. 15, no. 7
pp. 1134 – 1138

Abstract

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AIM: To explore the inhibiting effect of FTY720 on corneal neovascularization(CNV)of rat.METHODS: MTT assay and cells scratch were adopted to observe hyperplasia of human umbilical vein endothelial cells(HUVECs)and cell migration induced by sphingosine-1-phosphate(S1P)after using FTY720 of different concentration. The effect of FTY720 on CNV induced by S1P in a rat corneal micropocket model was detected. 30SD rats were randomly divided into group A, group B and group C with 10 rats per group. S1P and 0μg, 5μg, and 20μg FTY720 controlled-released particles were implanted into the corneal stroma. The growth of CNV and having pathological examination on 12d after the operation was observed. Findings was analyzed by one-way ANOVA.RESULTS: 10, 102, 103, and 104 nmol/L FTY720 and HUVECs co-incubate 72h could inhibit cell proliferation(P2(F=145.155, PPCONCLUSION: FTY720 can inhibit S1P-induced corneal neovascularization.

Published in Guoji Yanke Zazhi

ISSN: 1672-5123 (Print)
Publisher: Press of International Journal of Ophthalmology (IJO PRESS)
Country of publisher: China
LCC subjects: Medicine: Ophthalmology
Website: http://ies.ijo.cn/

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