Frontiers in Cell and Developmental Biology (May 2021)
Essential Role of CFAP53 in Sperm Flagellum Biogenesis
- Bingbing Wu,
- Bingbing Wu,
- Xiaochen Yu,
- Xiaochen Yu,
- Xiaochen Yu,
- Xiaochen Yu,
- Xiaochen Yu,
- Chao Liu,
- Lina Wang,
- Tao Huang,
- Tao Huang,
- Tao Huang,
- Tao Huang,
- Tao Huang,
- Gang Lu,
- Gang Lu,
- Gang Lu,
- Gang Lu,
- Gang Lu,
- Gang Lu,
- Zi-Jiang Chen,
- Zi-Jiang Chen,
- Zi-Jiang Chen,
- Zi-Jiang Chen,
- Zi-Jiang Chen,
- Zi-Jiang Chen,
- Wei Li,
- Wei Li,
- Hongbin Liu,
- Hongbin Liu,
- Hongbin Liu,
- Hongbin Liu,
- Hongbin Liu,
- Hongbin Liu
Affiliations
- Bingbing Wu
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing, China
- Bingbing Wu
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Xiaochen Yu
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Xiaochen Yu
- Key laboratory of Reproductive Endocrinology of the Ministry of Education, Shandong University, Jinan, China
- Xiaochen Yu
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Xiaochen Yu
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Xiaochen Yu
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, China
- Chao Liu
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing, China
- Lina Wang
- Department of Respiratory Medicine, National Clinical Research Center of Respiratory Diseases, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, China
- Tao Huang
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Tao Huang
- Key laboratory of Reproductive Endocrinology of the Ministry of Education, Shandong University, Jinan, China
- Tao Huang
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Tao Huang
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Tao Huang
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, China
- Gang Lu
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Gang Lu
- Key laboratory of Reproductive Endocrinology of the Ministry of Education, Shandong University, Jinan, China
- Gang Lu
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Gang Lu
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Gang Lu
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, China
- Gang Lu
- CUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong
- Zi-Jiang Chen
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Zi-Jiang Chen
- Key laboratory of Reproductive Endocrinology of the Ministry of Education, Shandong University, Jinan, China
- Zi-Jiang Chen
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Zi-Jiang Chen
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Zi-Jiang Chen
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, China
- Zi-Jiang Chen
- CUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong
- Wei Li
- State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing, China
- Wei Li
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China
- Hongbin Liu
- Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Jinan, China
- Hongbin Liu
- Key laboratory of Reproductive Endocrinology of the Ministry of Education, Shandong University, Jinan, China
- Hongbin Liu
- Shandong Key Laboratory of Reproductive Medicine, Jinan, China
- Hongbin Liu
- Shandong Provincial Clinical Research Center for Reproductive Health, Jinan, China
- Hongbin Liu
- National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, China
- Hongbin Liu
- CUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong
- DOI
- https://doi.org/10.3389/fcell.2021.676910
- Journal volume & issue
-
Vol. 9
Abstract
The sperm flagellum is essential for male fertility. Despite vigorous research progress toward understanding the pathogenesis of flagellum-related diseases, much remains unknown about the mechanisms underlying the flagellum biogenesis itself. Here, we show that the cilia and flagella associated protein 53 (Cfap53) gene is predominantly expressed in testes, and it is essential for sperm flagellum biogenesis. The knockout of this gene resulted in complete infertility in male mice but not in the females. CFAP53 localized to the manchette and sperm tail during spermiogenesis, the knockout of this gene impaired flagellum biogenesis. Furthermore, we identified two manchette and sperm tail-associated proteins that interacted with CFAP53 during spermiogenesis. Together, our results suggest that CFAP53 is an essential protein for sperm flagellum biogenesis, and its mutations might be associated with multiple morphological abnormalities of the flagella (MMAF).
Keywords
