Early Outcomes of Right Ventricular Pressure and Volume Overload in an Ovine Model
Hamida Al Hussein,
Hussam Al Hussein,
Marius Mihai Harpa,
Simina Elena Rusu Ghiragosian,
Simona Gurzu,
Bogdan Cordos,
Carmen Sircuta,
Alexandra Iulia Puscas,
David Emanuel Anitei,
Cynthia Lefter,
Horatiu Suciu,
Dan Simionescu,
Klara Brinzaniuc
Affiliations
Hamida Al Hussein
Doctoral School, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Hussam Al Hussein
Department of Anatomy and Embryology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Marius Mihai Harpa
Regenerative Medicine Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Simina Elena Rusu Ghiragosian
Department of Pediatrics III, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Simona Gurzu
Department of Morphopathology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Bogdan Cordos
Regenerative Medicine Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Carmen Sircuta
Department of Anesthesiology and Critical Care, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania
Alexandra Iulia Puscas
Department of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania
David Emanuel Anitei
Department of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania
Cynthia Lefter
Department of Anatomy and Embryology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Horatiu Suciu
Department of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania
Dan Simionescu
Regenerative Medicine Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Klara Brinzaniuc
Department of Anatomy and Embryology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540142 Targu Mures, Romania
Right ventricular (RV) failure is a common complication in multiple congenital heart disease (CHD), significantly increasing morbidity and mortality. Despite its impact, no therapies specifically target the failing RV. The growing population of CHD patients underscores the need to understand the pathophysiology of RV failure through preclinical research. This study aimed to develop an ovine model of RV failure induced by pressure and volume overload. A total of 14 juvenile sheep randomly underwent pulmonary artery banding (n = 6), pulmonary leaflet perforation (n = 4), and pulmonary annulotomy with transannular patching (TAP) (n = 4). Detailed anesthetic and surgical protocols were described, and intraoperative and early postoperative complications were evaluated. Acute RV pressure overload resulted in a 120% increase in RV pressure (p = 0.0312). The stroke volume index and cardiac index significantly declined (p = 0.0312), and there was a significant decrease in ScvO2 (p = 0.0312). Both TAP and leaflet perforation achieved moderate-to-severe pulmonary regurgitation. Both procedures led to an incremental trend in RV pressures and resulted in a 24% increase in the stroke volume index. All techniques demonstrated safety and feasibility, with low mortality. This comprehensive model could be reproducible in other large animal models, offering a robust platform for preclinical research into CHD-RV failure models.
