Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a

Ariya Khamwut; Juthamas Nimnual; Nantinee Chomta; Pattaraporn Nimsamer; Pattaraporn Nimsamer; Oraphan Mayuramart; Pornchai Kaewsapsak; Pornchai Kaewsapsak; Siripat Pasittungkul; Yong Poovorawan; Sunchai Payungporn; Sunchai Payungporn

doi:10.3389/ebm.2025.10387

Experimental Biology and Medicine (May 2025)

Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a

Ariya Khamwut,
Juthamas Nimnual,
Nantinee Chomta,
Pattaraporn Nimsamer,
Pattaraporn Nimsamer,
Oraphan Mayuramart,
Pornchai Kaewsapsak,
Pornchai Kaewsapsak,
Siripat Pasittungkul,
Yong Poovorawan,
Sunchai Payungporn,
Sunchai Payungporn

Affiliations

Ariya Khamwut: Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Juthamas Nimnual: Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Nantinee Chomta: Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Pattaraporn Nimsamer: Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Pattaraporn Nimsamer: Division of Medical Bioinformatics, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Oraphan Mayuramart: Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Pornchai Kaewsapsak: Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Pornchai Kaewsapsak: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Siripat Pasittungkul: Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Yong Poovorawan: Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Sunchai Payungporn: Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Sunchai Payungporn: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

DOI: https://doi.org/10.3389/ebm.2025.10387
Journal volume & issue: Vol. 250

Abstract

Read online

Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 102 and 103 copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.

Published in Experimental Biology and Medicine

ISSN: 1535-3702 (Print); 1535-3699 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Medicine
Website: https://www.ebm-journal.org/journals/experimental-biology-and-medicine

About the journal

Abstract

Keywords