Antimicrobial activity of silver nanoparticles encapsulated in poly-N-isopropylacrylamide-based polymeric nanoparticles

Abstract

Muhammad Qasim,1 Nopphadol Udomluck,1 Jihyun Chang,1 Hansoo Park,1 Kyobum Kim2 1School of Integrative Engineering, Chung-Ang University, Seoul, 2Division of Bioengineering, Incheon National University, Incheon, Republic of Korea Abstract: In this study, we analyzed the antimicrobial activities of poly-N-isopropylacrylamide (pNIPAM)-based polymeric nanoparticles encapsulating silver nanoparticles (AgNPs). Three sizes of AgNP-encapsulating pNIPAM- and pNIPAM-NH2-based polymeric nanoparticles were fabricated. Highly stable and uniformly distributed AgNPs were encapsulated within polymeric nanoparticles via in situ reduction of AgNO3 using NaBH4 as the reducing agent. The formation and distribution of AgNPs was confirmed by UV-visible spectroscopy, transmission electron microscopy, and inductively coupled plasma optical emission spectrometry, respectively. Both polymeric nanoparticles showed significant bacteriostatic activities against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria depending on the nanoparticle size and amount of AgNO3 used during fabrication. Keywords: pNIPAM, silver nanoparticles, antimicrobial activities, surface charge

Keywords

• pNIPAM
• Silver nanoparticles
• Antimicrobial activities
• Surface charge.