Response of Nitrate Metabolism in Seedlings of Oilseed Rape (Brassica napus L.) to Low Oxygen Stress

Abstract

In order to understand the response of nitrate metabolism in seedlings of oilseed rape (Brassica napus L.) to low oxygen stress (LOS), two cultivars were studied at different light, LOS time and exogenous nitrate concentrations under hydroponic stress. Results show that N-uptake and dry matter of rape seedlings were decreased after LOS stress while nitrate accumulation (NA) under LOS was induced by darkness. Nitrate accumulation peaked at 3 d while root activity (RA, defined as dehydrogenase activity) decreased with prolonged waterlogging exposure. Exogenous nitrate significantly elevated NA and RA. Tungstate (TS) and LOS inhibited nitrate reductase (NR) activity while NR transcription and activity were enhanced by exogenous nitrate. Low oxygen stress stimulated the activity of superoxide dismutase (SOD) and peroxidase (POD) slightly, but inhibited that of catalase (CAT). B. napus L. Zhongshuang 10 (ZS10), a LOS tolerant cultivar, displayed smaller decrease upon dry matter under LOS, higher NA in darkness and lower NA in light than B. napus L. Ganlan CC (GAC), a LOS sensitive variety. ZS10 had lower NA and higher RA after waterlogging and exogenous nitrate treatment, and higher NR activity under TS inhibition than GAC, but the activity of antioxidant enzymes did not change under LOS. The results indicate that nitrate metabolism involved tolerance of rape seedlings to LOS, with lower accumulation and higher reduction of nitrate being related to higher LOS tolerance of rape seedlings exposed to waterlogging.

Keywords

• Brassica napus
• low oxygen stress
• nitrate accumulation
• nitrate reductase
• gene expression