PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus

Abstract

Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.

Keywords

• influenza
• influenza virus
• viruses
• H7N9
• influenza A(H7N9) virus
• neuraminidase