The Anti-Virulence Effect of Vismia guianensis against Candida albicans and Candida glabrata

Elizangela Pestana Motta; Josivan Regis Farias; Arthur André Castro da Costa; Anderson França da Silva; Alberto Jorge Oliveira Lopes; Maria do Socorro Sousa Cartágenes; Roberto Nicolete; Afonso Gomes Abreu; Elizabeth Soares Fernandes; Flavia Raquel Fernandes Nascimento; Cláudia Quintino da Rocha; Cristina Andrade Monteiro; Rosane Nassar Meireles Guerra

doi:10.3390/antibiotics11121834

Antibiotics (Dec 2022)

The Anti-Virulence Effect of Vismia guianensis against Candida albicans and Candida glabrata

Elizangela Pestana Motta,
Josivan Regis Farias,
Arthur André Castro da Costa,
Anderson França da Silva,
Alberto Jorge Oliveira Lopes,
Maria do Socorro Sousa Cartágenes,
Roberto Nicolete,
Afonso Gomes Abreu,
Elizabeth Soares Fernandes,
Flavia Raquel Fernandes Nascimento,
Cláudia Quintino da Rocha,
Cristina Andrade Monteiro,
Rosane Nassar Meireles Guerra

Affiliations

Elizangela Pestana Motta: Laboratório de Imunofisiologia, Departamento de Patologia, Universidade Federal do Maranhão, Avenida dos Portugueses, 1966, Ensino Integrado, Bloco 1, São Luís 65080-805, MA, Brazil
Josivan Regis Farias: Laboratório de Imunofisiologia, Departamento de Patologia, Universidade Federal do Maranhão, Avenida dos Portugueses, 1966, Ensino Integrado, Bloco 1, São Luís 65080-805, MA, Brazil
Arthur André Castro da Costa: Laboratório de Imunofisiologia, Departamento de Patologia, Universidade Federal do Maranhão, Avenida dos Portugueses, 1966, Ensino Integrado, Bloco 1, São Luís 65080-805, MA, Brazil
Anderson França da Silva: Laboratório de Imunofisiologia, Departamento de Patologia, Universidade Federal do Maranhão, Avenida dos Portugueses, 1966, Ensino Integrado, Bloco 1, São Luís 65080-805, MA, Brazil
Alberto Jorge Oliveira Lopes: Laboratório Experimental de Estudos da Dor, Departamento de Ciências Fisiológicas, Universidade Federal do Maranhão, São Luís 65080-805, MA, Brazil
Maria do Socorro Sousa Cartágenes: Laboratório Experimental de Estudos da Dor, Departamento de Ciências Fisiológicas, Universidade Federal do Maranhão, São Luís 65080-805, MA, Brazil
Roberto Nicolete: Fiocruz Ceará-Rua São José, S/N-Precabura, Eusébio 61773-270, CE, Brazil
Afonso Gomes Abreu: Laboratóio de Patogenicidade Microbiana, Programa de Pós-Graduação em Biologia Microbiana, Universidade UNICEUMA, Rua Josué Montelo, 1-Renascença, São Luís 65075-120, MA, Brazil
Elizabeth Soares Fernandes: Instituto Pelé Pequeno Príncipe, Av. Silva Jardim, 1632-Água Verde, Curitiba 80250-060, PR, Brazil
Flavia Raquel Fernandes Nascimento: Laboratório de Imunofisiologia, Departamento de Patologia, Universidade Federal do Maranhão, Avenida dos Portugueses, 1966, Ensino Integrado, Bloco 1, São Luís 65080-805, MA, Brazil
Cláudia Quintino da Rocha: Laboratório de Química de Produtos Naturais, Centro de Ciências Exatas e Tecnológicas, Universidade Federal do Maranhão, São Luís 65080-805, MA, Brazil
Cristina Andrade Monteiro: Departamento de Biologia, Instituto Federal do Maranhão, Avenida Getúlio Vargas, No 4, Monte Castelo, São Luís 65030-005, MA, Brazil
Rosane Nassar Meireles Guerra: Laboratório de Imunofisiologia, Departamento de Patologia, Universidade Federal do Maranhão, Avenida dos Portugueses, 1966, Ensino Integrado, Bloco 1, São Luís 65080-805, MA, Brazil

DOI: https://doi.org/10.3390/antibiotics11121834
Journal volume & issue: Vol. 11, no. 12
p. 1834

Abstract

Read online

In folk medicine, Vismia guianensis is used to treat skin diseases and mycoses in the Amazon region. We evaluated the anti-Candida activity of the hydroalcoholic extract from the leaves of Vismia guianensis (EHVG). HPLC-PDA and FIA-ESI-IT-MSn were used to chemically characterize EHVG. The anti-Candida activity was determined in vitro by the minimum inhibitory concentrations (MIC) against Candida glabrata (ATCC-2001); Candida albicans (ATCC-90028, ATCC-14053, and ATCC-SC5314), and C. albicans clinical isolates. EHVG effects on adhesion, growth, and biofilm formation were also determined. Molecular docking was used to predict targets for EHVG compounds. The main compounds identified included anthraquinone, vismione D, kaempferol, quercetin, and vitexin. EHVG was fungicidal against all tested strains. C. albicans ATCC 14053 and C. glabrata ATCC 2001 were the most sensitive strains, as the extract inhibited their virulence factors. In silico analysis indicated that vismione D presented the best antifungal activity, since it was the most effective in inhibiting CaCYP51, and may act as anti-inflammatory and antioxidant agent, according to the online PASS prediction. Overall, the data demonstrate that EHVG has an anti-Candida effect by inhibiting virulence factors of the fungi. This activity may be related to its vismione D content, indicating this compound may represent a new perspective for treating diseases caused by Candida sp.

Published in Antibiotics

ISSN: 2079-6382 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.mdpi.com/journal/antibiotics

About the journal

Abstract

Keywords