Effect of salicylic acid and methyl jasmonate on the production of rosmarinic acid and caffeic acid in callus culture of Salvia lerrifolia Benth

Abstract

In this study, callus formation of Salvia leriifolia Benth by stem and leaf explants in the combination of NAA and 2,4-D with Kin and the effect of salicylic acid and methyl jasmonate elicitors (50, 100, 150 mM) in callus on some secondary metabolites were studied. NAA in combination with Kin had no callus production in both of stem and leaf explants, while the most appropriate callus produced by leaf explant at a concentration of 1 mg/L Kin with 2 mg/L 2,4-D. The highest fresh and dry weight, total phenol and flavonoids content were observed in calluses treated with 100 μM of methylisammonate, however, with increasing salicylic acid concentration, fresh and dry weight, total phenol content, flavonoids, rosmarinic acid increased, and the highest content of rusamicin acid and caffeic acid was observed in calluses treated with 100 and 150 μM salicylic acid, respectively. According to the results, it is stated that, by optimizing the concentrations of the ellicetors, it is possible to produce the desired secondary metabolites of salvia in in vitro conditions

Keywords

• Salvia leriifolia B
• Salicylic Acid
• methyl jasmonate
• rosemaric acid
• caffeic acid