Comparison the antimicrobial effect of methanolic total extracts and petroleum ether fractions of flowering aerial parts of Glaucium vitellinum Boiss. & Buhse and Gaillonia aucheri Jaub. & Spach

Abstract

Background: With increasing use of antibiotics, the number and type of microbial resistance to antibiotics have been raised and at result the need for synthesis of new antimicrobials or acquire new sources of antimicrobial is indisputable. In this study the antimicrobial effects of the flowering aerial parts of Glaucium vitellinum and Gaillonia aucheri were investigated.Materials and Methods: The antimicrobial effect of mentioned extracts against Escherichia coli PTCC 1399, Pseudomonas aeruginosa PTCC 1430, Salmonella typhimurium PTCC 1639, Staphylococcus aureus PTCC 1431 and Candida albicans PTCC 5027 were evaluated by disk diffusion and the microdilution method based on CLSI protocol 2012.Results: No any zone of inhibition were detected by disk diffusion method against tested microbes for Glaucium vitellinum. After deletion of agar interference, the minimal inhibitory concentration (MIC) was determined by broth microdilution method for two plants. None of tested extracts were effective against Gram negative tested bacteria except alkaloid fraction of G. vitellinum which was effective against S. typhi. In contrast, the extracts and fractions of both plans were effective against tested gram positive bacteria especially S. aureus.Conclusion: The best result of MIC was detected for alkaloid fraction of G. vitellinum (0.09 mg/ml) Vs. G. aucheri (125 mg/ml) against S.aureus.

Keywords

• Gaillonia aucheri, Glaucium vitellinum, antibacterial agents, antifungal agents, MIC