Violation of the conditions of animals keeping and the lack of proper level of veterinary and sanitary culture on farms does not allow to achieve a balance between the organism of the cattle and the environment of its stay. The uneven distribution of body weight on the surface of the sole caused by keeping animals on a rough floor promotes the emergence of producing corns, stretching of the tendons and ligaments, slippery flooring leads to the fall of animals and the occurrence of various injuries, in particular, fractures of the limbs, tensile bond, capsule of the joint, excessively hard floor – to excessive erosion of the horn of the sole, etc. In such herds it is difficult to maintain animal health at the proper level and prevent the occurrence of, in particular, aseptic pododermatitis. The article deals with the results on the dissemination of aseptic pododermatitis in cows for the presence of animals on wooden and reinforced concrete slit floors during the winter and standstill period of containment. It has been established that aseptic pododermatitis in cows on reinforced concrete slit floor is registered in 71.6% of cases, while on wooden – only 28.4%. In the hoof horn of the thoracic and pelvic extremities of the cows, with aseptic pododermatitis, the amount of moisture decreased by 8.2 and 9.3% respectively, the concentration of SH-groups increases by 10.6 and 15.8%, with a possible decrease in the content of calcium, sulfur, cuprum and zinc; decreases the hoof horn density and resistance to abrasion of the epithelium of the hooves of the pelvic limbs by 1.6 and 18.0%, as well as the intensity of the abrasion sole hooves of the thoracic and pelvic limbs is increased by 7.5 and 20.0%, which helps to reduce the growth of the epidermis of the sole. Consequently, changes in certain biochemical and biophysical indices of hoof horns of cows suffering from aseptic pododermatitis, which were held on the reinforced concrete slit, indicate a deterioration in the quality of the epidermal hooves and excessive erosion of the sole, which is one of the causes of the occurrence and development of aseptic pododermatitis in cows.