A behavioral tagging account of kinase contribution to memory formation after spaced aversive training
Pablo Budriesi,
Ramiro Tintorelli,
Julieta Correa,
Maria Eugenia Villar,
Paul Marchal,
Martin Giurfa,
Haydee Viola
Affiliations
Pablo Budriesi
Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA-CONICET, Ciudad Autónoma de Buenos Aires, Argentina
Ramiro Tintorelli
Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA-CONICET, Ciudad Autónoma de Buenos Aires, Argentina
Julieta Correa
Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA-CONICET, Ciudad Autónoma de Buenos Aires, Argentina
Maria Eugenia Villar
Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA-CONICET, Ciudad Autónoma de Buenos Aires, Argentina; Departamento de Biología y Geología, Física y Química Inorgánica, Área de Biodiversidad y Conservación, Universidad Rey Juan Carlos, Madrid, Spain
Paul Marchal
Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA-CONICET, Ciudad Autónoma de Buenos Aires, Argentina; Poe Lab, Integrative Biology and Physiology department, University of California Los Angeles, Los Angeles, CA, USA
Martin Giurfa
Centre de Recherches sur la Cognition Animale, Centre de Biologie Intégrative (CBI), University of Toulouse, CNRS, UPS, 31062 Toulouse cedex 9, France; Institut Universitaire de France (IUF), Paris, France
Haydee Viola
Instituto de Biología Celular y Neurociencia “Prof. E. De Robertis” (IBCN), Facultad de Medicina, UBA-CONICET, Ciudad Autónoma de Buenos Aires, Argentina; Departamento de Fisiología, Biología Molecular y Celular “Dr. Héctor Maldonado” (FBMC), Facultad de Ciencias Exactas y Naturales, UBA, Ciudad Autónoma de Buenos Aires, Argentina; Instituto Tecnológico de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina; Corresponding author
Summary: Long-term memory (LTM) can be induced by repeated spaced training trials. Using the weak inhibitory avoidance (wIA) task, we showed that one wIA session does not lead to a 24-h LTM, whereas two identical wIA sessions spaced by 15 min to 6 h induce a 24-h LTM. This LTM promotion depends both on hippocampal protein synthesis and the activity of several kinases. In agreement with the behavioral tagging (BT) hypothesis, our results suggest that the two training sessions induce transient learning tags and lead, via a cooperative effect, to the synthesis of plasticity-related proteins (PRPs) that become available and captured by the tag from the second session. Although ERKs1/2 are needed for PRPs synthesis and CaMKs are required for tag setting, PKA participates in both processes. We conclude that the BT mechanism accounts for the molecular constraints underlying the classic effect of spaced learning on LTM formation.
